Gonkowski Ignacy, Gonkowski Slawomir, Dzika Ewa, Wojtkiewicz Joanna
Students' Scientific Club of Pathophysiologists, Department of Human Physiology and Pathophysiology, School of Medicine, University of Warmia and Mazury, 10-082 Olsztyn, Poland.
Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str. 13, 10-718 Olsztyn, Poland.
Toxics. 2020 Dec 28;9(1):1. doi: 10.3390/toxics9010001.
Calbindin D-28k (CB) is a calcium-binding protein widely distributed in living organisms that may act as a calcium buffer and sensory protein. CB is present in the enteric nervous system (ENS) situated in the gastrointestinal tract, which controls the majority of activities of the stomach and intestine. The influence of various doses of bisphenol A (BPA)-a chemical compound widely used in plastics production-on the number and distribution of CB-positive enteric neuronal cells in the porcine caecum was investigated with an immunofluorescence technique. The obtained results showed that low dosages of BPA resulted in an increase in the number of CB-positive neuronal cells in the myenteric (MP) and inner submucous (ISP) plexuses, whereas it did not alter the number of such neuronal cells in the outer submucous plexus (OSP). High dosages of BPA caused the increase in the amount of CB-positive perikarya in all the above-mentioned kinds of the caecal neuronal plexuses. These observations strongly suggest that CB in the ENS participates in the processes connected with the toxic activity of BPA. Most likely, the changes noted in this experiment result from the adaptive and protective properties of CB.
钙结合蛋白D-28k（CB）是一种广泛分布于生物体内的钙结合蛋白，可能作为钙缓冲蛋白和传感蛋白发挥作用。CB存在于胃肠道的肠神经系统（ENS）中，该系统控制着胃和肠道的大部分活动。采用免疫荧光技术研究了不同剂量的双酚A（BPA，一种广泛用于塑料生产的化合物）对猪盲肠中CB阳性肠神经元细胞数量和分布的影响。所得结果表明，低剂量的BPA导致肌间神经丛（MP）和内黏膜下神经丛（ISP）中CB阳性神经元细胞数量增加，而外黏膜下神经丛（OSP）中此类神经元细胞数量未发生改变。高剂量的BPA导致上述所有类型的盲肠神经丛中CB阳性核周体数量增加。这些观察结果强烈表明，ENS中的CB参与了与BPA毒性活动相关的过程。很可能，本实验中观察到的变化是由CB的适应性和保护特性引起的。
