Lamba Saini Monika, Weynand Birgit, Rahier Jacques, Mourad Michel, Hamoir Marc, Marbaix Etienne
Anatomie pathologique, Cliniques universitaires Saint- Luc, Université catholique de Louvain, Avenue Hippocrate, 10/T-1, Brussels, 1200, Belgium.
Anatomie pathologique, CHU Dinant-Godinne, Université catholique de Louvain, Avenue Docteur G. Thérasse, 1, Yvoir, 5530, Belgium.
Diagn Pathol. 2015 Apr 18;10:32. doi: 10.1186/s13000-015-0262-8.
Encapsulated follicular tumours with equivocal papillary thyroid carcinoma (PTC) type nuclear features continue to remain a challenge despite the recent attempts to classify these borderline lesions. The term 'well differentiated tumour of uncertain malignant potential (WDT-UMP)' was introduced to classify these tumours. The present study aimed to evaluate the role of a cell cycle regulator like cyclin D1 in these tumours along with assessment of other well established PTC markers like galectin-3, HBME-1, CK19.
Thirteen cases of metastatic PTC, papillary microcarcinoma and follicular variant of PTC (FVPTC) were identified from a histological review of 510 cases. In addition, 13 cases of a subset of follicular adenomatoid nodules with focal areas showing nuclear features characteristic of PTC, identified as WDT-UMP, were also analyzed. Immunohistochemical analysis of galectin-3, HBME-1, CK19 and the proliferation markers Ki67 and cyclin D1 was performed. Lesions were analyzed for cyclin D1 gene amplification by fluorescent in-situ hybridization.
All WDT-UMP lesions showed immunolabelling of cyclin D1, Ki67; 11/ 13 cases showed immunolabelling of CK19; 10/13 cases showed immunolabelling of HBME-1 and 4/13 cases showed immunolabelling of galectin-3. Surrounding benign adenomatoid areas showed no to faint focal staining in all thirteen cases of cyclin D1, HBME-1 and galectin-3. A low rate of cyclin D1 gene amplification was identified in a significant proportion of cells in the WDT-UMP lesions as compared to surrounding benign adenomatoid areas.
Increased expression of cyclin D1 and amplification of its gene along with immunolabelling of HBME-1 in WDT-UMP lesions showing cytological features of papillary thyroid carcinoma within follicular adenomatoid nodules suggest that these areas could correspond to a precursor lesion of follicular variant of PTC. Overexpression of cyclin D1, associated with the amplification of the gene suggests that these WDT-UMP lesions are an intermediate between the benign and malignant groups making this group of lesions a reliable precursor of FVPTC.
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1851820807142117.
尽管最近尝试对这些临界性病变进行分类，但具有不明确的乳头状甲状腺癌（PTC）核特征的包膜滤泡性肿瘤仍然是一个挑战。引入了“恶性潜能不确定的高分化肿瘤（WDT-UMP）”这一术语来对这些肿瘤进行分类。本研究旨在评估细胞周期调节因子如细胞周期蛋白D1在这些肿瘤中的作用，并评估其他已确立的PTC标志物如半乳糖凝集素-3、HBME-1、细胞角蛋白19（CK19）。
通过对510例病例的组织学回顾，确定了1
