Zhang Zai-Bao, Guan Qian, Zhang Zhen-Hai
State Key Laboratory for Organ Failure Research//National Clinical Research Center for Kidney Disease//Renal Division, Nanfang Hospital, Southern Medical University, Guangzhou 510515 China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2015 Apr;35(4):463-7.
Antibody, the major effector in adaptive immunity, plays key roles in protective and pathogenic immune responses. Integrative analyses of antibody development, differentiation, and maturation promote the research in immune mechanism, vaccine design, and therapies for autoimmune disorders. The development of next generation sequencing technologies has enabled large-scale characterization of functional antibody repertoires. With the advantages of next generation sequencing, antibody and antibody repertoire analysis have been successfully used in identification of HIV-1-broadly neutralizing antibodies, design of rationale structure-based vaccine, and development of immunology. With increasing sequence length and precision, improvement of experimental protocols and bioinformatics analyses, and development of single cell sequencing technology, antibody repertoire sequencing will expedite the research in antibody-related immune response, and thus facilitates vaccine design for infectious diseases, clinical diagnosis and interference of autoimmune diseases. This review introduces the technologies, progresses, applications, and caveats of antibody repertoire sequencing.
抗体作为适应性免疫中的主要效应因子，在保护性和致病性免疫反应中发挥关键作用。对抗体发育、分化和成熟的综合分析推动了免疫机制、疫苗设计以及自身免疫性疾病治疗等方面的研究。新一代测序技术的发展使得大规模表征功能性抗体库成为可能。凭借新一代测序的优势，抗体及抗体库分析已成功应用于HIV-1广谱中和抗体的鉴定、基于合理结构的疫苗设计以及免疫学发展。随着序列长度和精度的增加、实验方案和生物信息学分析的改进以及单细胞测序技术的发展，抗体库测序将加速与抗体相关的免疫反应研究，从而促进传染病疫苗设计、自身免疫性疾病的临床诊断和干预。本文综述了抗体库测序的技术、进展、应用及注意事项。
