a Institute of Animal Husbandry and Veterinary Science , Zhejiang Academy of Agricultural Sciences , Hangzhou , Zhejiang , P. R. China.
Arch Anim Nutr. 2015;69(3):201-11. doi: 10.1080/1745039X.2015.1034828. Epub 2015 Apr 24.
To determine the transient effects of weaning on the small intestine, 16 piglets were slaughtered at days 0, 1, 4 and 7 after weaning. Jejunal samples were collected to examine different enzyme activities and mRNA expressions of two stress protein families, namely, heat-shock proteins (HSP) and trefoil factors (TFF). Results showed that the activities of ceruloplasmin, alkaline phosphatase and lactate dehydrogenase, were significantly changed at Day 1 and/or Day 4. The mRNA expressions of HSP10, HSP60 and HSP90 showed a pattern of increased expression with time after weaning. Expression significantly differed between Day 0 and Day 7 after weaning. The mRNA expression of HSP70 was significantly increased on Day 1 only. Similarly, the mRNA expressions of TFF1 and TFF2 were significantly increased on Day 7 compared with those on Day 0. Expression of TFF3 was not affected by time after weaning. In conclusion, the present study indicated that weaning induced transient injury to small intestinal morphology and function. Particularly it changed enzyme activities and gene expression of stress proteins in the small intestine of piglets. At first time, a change in the gene expression of HSP10 and a gene overexpression of TFF1 in the small intestine of piglets after weaning was found.
为了确定断奶对小肠的瞬时影响，在断奶后第 0、1、4 和 7 天处死了 16 头仔猪。采集空肠样本以检查两种应激蛋白家族（热休克蛋白（HSP）和三叶因子（TFF））的不同酶活性和 mRNA 表达。结果表明，铜蓝蛋白、碱性磷酸酶和乳酸脱氢酶的活性在第 1 天和/或第 4 天发生明显变化。HSP10、HSP60 和 HSP90 的 mRNA 表达呈现出断奶后随时间增加的模式。断奶后第 0 天和第 7 天的表达差异显著。HSP70 的 mRNA 表达仅在第 1 天显著增加。同样，TFF1 和 TFF2 的 mRNA 表达在第 7 天与第 0 天相比显著增加。TFF3 的表达不受断奶后时间的影响。总之，本研究表明，断奶导致小肠形态和功能发生短暂损伤。特别是，它改变了仔猪小肠中应激蛋白的酶活性和基因表达。首次发现断奶后仔猪小肠中 HSP10 的基因表达变化和 TFF1 的基因过表达。
