Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
PLoS One. 2018 Jul 19;13(7):e0200680. doi: 10.1371/journal.pone.0200680. eCollection 2018.
When Lambs are weaned off ewe's milk, metabolic, structural, and functional changes often occur in the small intestine. Because information on the effects of weaning stress on the proteome of the intestine is limited, an animal model was established with eight pairs of twin lambs divided into artificially reared and ewe-reared groups, which was followed by proteome analysis using iTRAQ technology. Changes occurred in the morphology of the intestine and 5,338 proteins in three biological replicates with less than a 1.2% false discovery rate were identified and quantified. Among them, a subset of 389 proteins were screened as significantly up- (143) and down-regulated (246) in artificially reared compared with ewe-reared. According to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, the differentially expressed proteins that were strongly down-regulated were enriched in immune system processes, biological adhesion, and metabolic processes. The up-regulated proteins were enriched in gene expression, cellular biosynthetic processes, ribosome and RNA binding in response to weaning stress. A series of proteins associated with intestine morphology and immune function were identified, and levels of the mRNAs encoding these proteins were analyzed by real-time quantitative reverse transcription PCR. The results of this study increased our understanding of the response of lambs weaned off ewe's milk and helped to determine the mechanisms underlying weaning stress.
当羔羊断奶时，小肠通常会发生代谢、结构和功能变化。由于断奶应激对肠道蛋白质组影响的信息有限，因此建立了一个动物模型，将 8 对双胞胎羔羊分为人工饲养组和母羊饲养组，然后使用 iTRAQ 技术进行蛋白质组分析。在三个生物学重复中，形态发生变化，有 5338 种蛋白质的变化小于 1.2%的假发现率，这些蛋白质被鉴定和定量。其中，有 389 种蛋白质被筛选为人工饲养组与母羊饲养组相比明显上调（143 种）和下调（246 种）。根据基因本体论和京都基因与基因组百科全书途径富集分析，强烈下调的差异表达蛋白在免疫系统过程、生物粘附和代谢过程中富集。上调的蛋白在基因表达、细胞生物合成过程、核糖体和 RNA 结合方面富集，以响应断奶应激。确定了一系列与肠道形态和免疫功能相关的蛋白质，并通过实时定量逆转录 PCR 分析了这些蛋白质编码基因的 mRNA 水平。这项研究增加了我们对羔羊断奶的反应的理解，并有助于确定断奶应激的机制。
