Cong Lin, Zhu Yue, Tu Guanjun
Department of Orthopeadics, the First Hospital, China Medical University, Shenyang 110001, China. Email:
Department of Orthopeadics, the First Hospital, China Medical University, Shenyang 110001, China.
Zhonghua Wai Ke Za Zhi. 2015 Feb;53(2):116-20.
To study the expression of Aggrecan and the relationship between the variable number of tandem repeats (VNTR) of Aggrecan and lumbar disc herniation (LDH).
The disease group comprised of 74 patients already diagnosed with symptomatic LDH. The control group consisted of 15 patients restricted to spinal trauma and 113 healthy blood donors without symptoms of LDH who were not diagnosed with LDH. Disc tissue samples were obtained from surgical operations and blood samples were donated from all participants. The Aggrecan expression in isolated tissues was assessed by western blot using specific antibodies. The Aggrecan gene VNTR region was analyzed by PCR.
The Aggrecan expression positive rate of control group was statistically and significantly higher (control group:86.67%, disease group:13.51%;χ(2) = 34.83, P < 0.05) than that of the disease group. Moreover, there was a statistically significant higher frequency of Allele 25 or Allele 21 in disease group compared to controls (A25disease group = 22.97%, A25control group = 12.11%, χ(2)A25 = 8.20, PA25 = 0.004; A21disease group = 6.76%, A21control group = 0.39%, χ(2)A21 = 14.35, PA21 = 0.000). Compared to the participants with 2 Alleles>25 repeats, subjects with 1 or 2 Alleles ≤ 25 repeats statistically and significantly over represented the disease group without the expression of Aggrecan (χ(2) = 5.69, P = 0.017).
The findings suggest a relationship between Aggrecan and symptomatic LDH, where symptomatic LDH has a tendency of allele 21 and allele 25 repeats.In addition, an association between the distribution of Aggrecan gene VNTR polymorphism and the expression of Aggrecan is observed in symptomatic LDH.
研究聚集蛋白聚糖（Aggrecan）的表达以及Aggrecan串联重复序列可变数目（VNTR）与腰椎间盘突出症（LDH）之间的关系。
疾病组由74例已确诊为有症状LDH的患者组成。对照组包括15例脊柱外伤患者和113例无LDH症状且未被诊断为LDH的健康献血者。椎间盘组织样本取自手术操作，所有参与者均捐献血液样本。使用特异性抗体通过蛋白质印迹法评估分离组织中Aggrecan蛋白的表达。通过聚合酶链反应（PCR）分析Aggrecan基因的VNTR区域。
对照组Aggrecan蛋白表达阳性率在统计学上显著高于疾病组（对照组：86.67%，疾病组：13.51%；χ(2)=34.83，P<0.05）。此外，与对照组相比，疾病组中25等位基因或21等位基因的频率在统计学上显著更高（疾病组A25=22.97%，对照组A25=12.11%，χ(2)A25=8.20，PA25=0.004；疾病组A21=6.76%，对照组A21=0.39%，χ(2)A21=14.35，PA21=0.000）。与具有2个大于25次重复等位基因的参与者相比，具有1个或2个小于等于25次重复等位基因的受试者在疾病组中在统计学上显著过度代表了未表达Aggrecan蛋白的情况（χ(2)=5.69，P=0.017）。
研究结果表明Aggrecan与有症状的LDH之间存在关联，其中有症状的LDH具有21等位基因和25等位基因重复的倾向。此外，在有症状的LDH中观察到Aggrecan基因VNTR多态性的分布与Aggrecan蛋白表达之间存在关联。
