Vázquez-Jiménez Flor Elizabeth, Torres Javier, Flores-Luna Lourdes, Cerezo Silvia Giono, Camorlinga-Ponce Margarita
Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias UMAE, Hospital de Pediatria, IMSS., México, DF, Mexico.
Instituto Nacional de Salud Pública, México, Morelos, Mexico.
Helicobacter. 2016 Feb;21(1):60-8. doi: 10.1111/hel.12230. Epub 2015 Apr 24.
Adherence to the gastric epithelium is one of the most important steps of Helicobacter pylori to remain and cause disease. The aim of this study was to analyze whether H. pylori isolates from patients with different gastroduodenal diseases present differences in the pattern of adherence to gastric epithelial cells (AGS), in the ability to induce IL-8, and in the presence of virulence genes.
We tested 75 H. pylori strains isolated from nonatrophic gastritis, gastric cancer, and duodenal ulcer patients. The adhesion pattern and IL-8 induction were determined in AGS cells, and invasion of AGS cells was studied using a gentamicin protection assay. The IL-8 levels induced were determined by ELISA.
Helicobacter pylori strains presented diffuse adherence (DA) and localized (LA) adherence patterns, similar to those described for enteropathogenic E. coli (EPEC), were observed in AGS cells. A DA pattern was observed in 57% and LA in 43% of the strains, and DA was more frequent in isolates from patients with gastric cancer (p = 0.044). Strains with a LA pattern induced higher levels of IL-8 (p = 0.042) in AGS cells.
The adherence pattern was not associated with neither invasiveness nor with the presence of virulence genes. Our study shows that H. pylori strains present adherence patterns to AGS cells resembling those observed in EPEC and that these patterns may be associated with disease and with activity on AGS cells.
幽门螺杆菌黏附于胃上皮细胞是其持续存在并引发疾病的最重要步骤之一。本研究旨在分析从不同胃十二指肠疾病患者中分离出的幽门螺杆菌菌株在黏附胃上皮细胞（AGS）的模式、诱导白细胞介素-8（IL-8）的能力以及毒力基因的存在方面是否存在差异。
我们检测了从非萎缩性胃炎、胃癌和十二指肠溃疡患者中分离出的75株幽门螺杆菌菌株。在AGS细胞中测定黏附模式和IL-8诱导情况，并使用庆大霉素保护试验研究AGS细胞的侵袭情况。通过酶联免疫吸附测定法（ELISA）测定诱导的IL-8水平。
在AGS细胞中观察到幽门螺杆菌菌株呈现弥漫性黏附（DA）和局限性黏附（LA）模式，类似于肠道致病性大肠杆菌（EPEC）所描述的模式。57%的菌株观察到DA模式，43%的菌株观察到LA模式，DA在胃癌患者分离株中更常见（p = 0.044）。具有LA模式的菌株在AGS细胞中诱导更高水平的IL-8（p = 0.042）。
黏附模式与侵袭性和毒力基因的存在均无关。我们的研究表明，幽门螺杆菌菌株对AGS细胞的黏附模式类似于在EPEC中观察到的模式，并且这些模式可能与疾病以及对AGS细胞的活性有关。
