使用光稳定的聚集诱导发光发光团进行长期双光子神经成像。
Long-term two-photon neuroimaging with a photostable AIE luminogen.
作者信息
Qian Jun, Zhu Zhenfeng, Leung Chris Wai Tung, Xi Wang, Su Liling, Chen Guangdi, Qin Anjun, Tang Ben Zhong, He Sailing
机构信息
State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies; JORCEP (Sino-Swedish Joint Research Center of Photonics), Zhejiang University, 310058 Hangzhou, China.
Department of Chemistry, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, China.
出版信息
Biomed Opt Express. 2015 Mar 24;6(4):1477-86. doi: 10.1364/BOE.6.001477. eCollection 2015 Apr 1.
In neuroscience, fluorescence labeled two-photon microscopy is a promising tool to visualize ex vivo and in vivo tissue morphology, and track dynamic neural activities. Specific and highly photostable fluorescent probes are required in this technology. However, most fluorescent proteins and organic fluorophores suffer from photobleaching, so they are not suitable for long-term imaging and observation. To overcome this problem, we utilize tetraphenylethene-triphenylphosphonium (TPE-TPP), which possesses aggregation-induced emission (AIE) and two-photon fluorescence characteristics, for neuroimaging. The unique AIE feature of TPE-TPP makes its nanoaggregates resistant to photobleaching, which is useful to track neural cells and brain-microglia for a long period of time. Two-photon fluorescence of TPE-TPP facilitates its application in deep in vivo neuroimaging, as demonstrated in the present paper.
在神经科学领域，荧光标记双光子显微镜是一种很有前景的工具，可用于观察离体和活体组织形态，并追踪动态神经活动。该技术需要特异性强且光稳定性高的荧光探针。然而，大多数荧光蛋白和有机荧光团都存在光漂白问题，因此不适用于长期成像和观察。为克服这一问题，我们利用具有聚集诱导发光（AIE）和双光子荧光特性的四苯乙烯-三苯基鏻（TPE-TPP）进行神经成像。TPE-TPP独特的AIE特性使其纳米聚集体具有抗光漂白能力，这对于长时间追踪神经细胞和脑小胶质细胞很有用。如本文所示，TPE-TPP的双光子荧光有助于其在体内深部神经成像中的应用。
Zotero 插件