摩洛哥代谢综合征患者载脂蛋白A5基因的单倍型分析。
Haplotype analysis of the Apolipoprotein A5 gene in Moroccan patients with the metabolic syndrome.
作者信息
Ajjemami Maria, Ouatou Sanaa, Charoute Hicham, Fakiri Malika, Rhaissi Houria, Benrahma Houda, Rouba Hassan, Barakat Abdelhamid
机构信息
Département de Recherche Scientifique, Laboratoire de Génétique Moléculaire Humaine, Institut Pasteur du Maroc, 1, Place Louis Pasteur, 20360 Casablanca, Morocco.
Département de Recherche Scientifique, Laboratoire de Génétique Moléculaire Humaine, Institut Pasteur du Maroc, 1, Place Louis Pasteur, 20360 Casablanca, Morocco ; Univ Hassan 1, Laboratoire Agroalimentaire et Santé, 26000 Settat, Morocco.
出版信息
J Diabetes Metab Disord. 2015 Apr 16;14:29. doi: 10.1186/s40200-015-0160-3. eCollection 2015.
BACKGROUND
In this case-control study we investigated the relative contribution of commons APOA5 polymorphisms and haplotypes to the risk of metabolic syndrome in Moroccan patients.
METHODS
Using the International Diabetes Federation (IDF) criteria for metabolic syndrome, the study included 176 patients and 105 controls. We genotyped APOA5 polymorphisms (-1131 T > C, c.56C > G, c.553G > T and c.1259 T > C) by PCR-RFLP analysis. The effects of APOA5 polymorphisms and constructed haplotypes on metabolic syndrome were estimated using logistic regression analyses.
RESULTS
The statistical analysis showed a significant association between APOA5 -1131 T > C and APOA5 c.56C > G polymorphisms with metabolic syndrome in both Codominant and Dominant models. The APOA5 -1131 T > C polymorphism was associated with increased fasting glucose (p = 0.0295) and reduced HDL levels (p = 0.0091). Carriers of the APOA5 c.56G allele had increased triglyceride levels (p = 0.0435) and waist circumference (p = 0.0122). Similarly the APOA5 1259 T > C variant was associated with increased waist circumference (p = 0.0463). The haplotypes CCGT (OR = 3.223; p = 0.00278) and CGGT (OR = 8.234; p = 0.00534) were significantly associated with susceptibility to metabolic syndrome.
CONCLUSIONS
Our results confirms the association of APOA5 -1131 T > C and c.56C > G variants with the predisposition to metabolic syndrome complications.
背景
在这项病例对照研究中，我们调查了常见的载脂蛋白A5（APOA5）基因多态性和单倍型对摩洛哥患者代谢综合征风险的相对影响。
方法
采用国际糖尿病联盟（IDF）代谢综合征标准，该研究纳入了176例患者和105例对照。我们通过聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）对APOA5基因多态性（-1131T＞C、c.56C＞G、c.553G＞T和c.1259T＞C）进行基因分型。使用逻辑回归分析评估APOA5基因多态性和构建的单倍型对代谢综合征的影响。
结果
统计分析显示，在共显性和显性模型中，APOA5 -1131T＞C和APOA5 c.56C＞G基因多态性与代谢综合征之间存在显著关联。APOA5 -1131T＞C基因多态性与空腹血糖升高（p = 0.0295）和高密度脂蛋白水平降低（p = 0.0091）相关。APOA5 c.56G等位基因携带者的甘油三酯水平升高（p = 0.0435）和腰围增加（p = 0.0122）。同样，APOA5 1259T＞C变异与腰围增加相关（p = 0.0463）。单倍型CCGT（OR = 3.223；p = 0.00278）和CGGT（OR = 8.234；p = 0.00534）与代谢综合征易感性显著相关。
结论
我们的结果证实了APOA5 -1131T＞C和c.56C＞G变异与代谢综合征并发症易感性之间的关联。
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