2型糖尿病肾病患者中APOA5基因启动子区域-1131T>C多态性（rs662799）与血浆甘油三酯水平的关联
Association of APOA5 Gene Promoter Region -1131T>C Polymorphism (rs662799) to Plasma Triglyceride Level in Patients with Type 2 Diabetic Nephropathy.
作者信息
Mahrooz Abdolkarim, Zargari Mehryar, Ansari Vahid, Makhlough Atieh, Hashemi-Sooteh Mohammad-Bagher
机构信息
Faculty of Medicine, Department of Biochemistry, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences , Sari, Iran .
Faculty, Department of Nephrology, Imam Khomeini Hospital, Mazandaran University of Medical Sciences , Sari, IR Iran .
出版信息
J Clin Diagn Res. 2016 May;10(5):BC09-13. doi: 10.7860/JCDR/2016/19212.7895. Epub 2016 May 1.
INTRODUCTION
Diabetic Nephropathy (DN), a serious complication of Type 2 Diabetic Mellitus (T2DM), is progressive and susceptibility to DN varies among T2DM patients. ApoA5-1131T>C polymorphism revealed that is strongly associated with triglyceride levels and proposed as a predisposing factor for DN.
AIM
The purpose of this study was to investigate the association -1131T>C ApoA5 gene polymorphism with serum lipids levels in Type 2 diabetic (DM) patients with or without DN in north of Iran (Mazandaran province).
MATERIALS AND METHODS
This study comprised patients with established T2DM (n=161) and controls (n=58). Genotyping of APOA5 -1131T>C polymorphisms was performed by PCR-RFLP. Diabetic patients were divided into two groups: with nephropathy (DN+, n = 90) and without nephropathy (DN-, n = 71). Lipids and lipoproteins were assessed by enzymatic methods.
RESULTS
The genotype frequencies were 63.8 % TT, 31 % TC, 5.2 % CC in controls, 33.8% TT, 52.1 % TC, 14.1 % CC in DN- and 44.4 % TT, 36.7 % TC, 18.9 % CC in DN+ patients. The TC genotype and the CC genotype were overexpressed among DN+ and DN-population in comparison to the control group. The highest and the lowest TG levels in both diabetic patients and controls belonged to CC+TC and TT genotypes, respectively. Furthermore in both patients TG increased with this order: TT< TC<CC.
CONCLUSION
These results suggest that APOA5 -1131T>C polymorphisms influence lipid levels in type 2 diabetic patients.
引言
糖尿病肾病（DN）是2型糖尿病（T2DM）的一种严重并发症，具有进展性，且T2DM患者对DN的易感性存在差异。载脂蛋白A5（ApoA5）-1131T>C多态性显示与甘油三酯水平密切相关，并被认为是DN的一个易感因素。
目的
本研究旨在调查伊朗北部（马赞德兰省）有或无DN的2型糖尿病（DM）患者中ApoA5基因-1131T>C多态性与血脂水平的关联。
材料与方法
本研究纳入确诊的T2DM患者（n = 161）和对照组（n = 58）。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对APOA5 -1131T>C多态性进行基因分型。糖尿病患者分为两组：有肾病组（DN+，n = 90）和无肾病组（DN-，n = 71）。采用酶法评估血脂和脂蛋白水平。
结果
对照组中基因型频率分别为：TT 63.8%、TC 31%、CC 5.2%；DN-组中分别为：TT 33.8%、TC 52.1%、CC 14.1%；DN+组中分别为：TT 44.4%、TC 36.7%、CC 18.9%。与对照组相比，TC基因型和CC基因型在DN+组和DN-组中均过度表达。糖尿病患者和对照组中，TG水平最高和最低的分别属于CC+TC基因型和TT基因型。此外，两组患者的TG水平均按TT<TC<CC的顺序升高。
结论
这些结果表明，APOA5 -1131T>C多态性影响2型糖尿病患者的血脂水平。
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