Sait Sabrina, Hamri-Zeghichi Sabrina, Boulekbache-Makhlouf Lila, Madani Khodir, Rigou Peggy, Brighenti Virginia, Pio Prencipe Francesco, Benvenuti Stefania, Pellati Federica
Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria; Département de Biologie, Faculté des Sciences, Université M'Hamed Bougara, Boumerdès 10 035000, Algeria.
Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria.
J Pharm Biomed Anal. 2015;111:231-40. doi: 10.1016/j.jpba.2015.03.027. Epub 2015 Apr 3.
Paronychia argentea Lam., belonging to the Caryophyllaceae family, is a perennial plant widely distributed in Algeria. Even though this plant is used in the Algerian popular medicine, its phytochemical characterization is incomplete. In this study, the flavonoid profile and the in vitro antioxidant activity of the ethanolic extract, decoction and infusion of P. argentea aerial parts are reported. Flavonoids were analyzed by means of high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry. Eleven compounds were identified and six of them, including isorhamnetin-3-O-dihexoside, quercetin-3-O-glucoside, quercetinmethylether-O-hexoside, quercetin, jaceosidin and isorhamnetin, were described in this plant for the first time. The ethanol extract showed the highest flavonoid content, followed by the decoction and the infusion (25.4 ± 0.8 mg/g of DM, 8.4 ± 0.5 mg/g of DM, 0.2 mg/g of DM, respectively), while the best antioxidant activity was shown by the decoction (RC0.5 = 178 μg/mL for reducing power, 72.4% of inhibition of lipid peroxidation, IC50 = 27.38μ g/mL for DPPH radical scavenging activity and 59.7% of inhibition of NO radical). These results showed that P. argentea decoction could be considered as a valuable source of flavonoids and antioxidants that might contribute to the valorization of the phytotherapeutic potential of this plant.
银色指甲草（Paronychia argentea Lam.）属于石竹科，是一种广泛分布于阿尔及利亚的多年生植物。尽管这种植物在阿尔及利亚民间医学中有所应用，但其植物化学特征尚不完整。本研究报道了银色指甲草地上部分乙醇提取物、煎剂和浸剂的黄酮类成分及其体外抗氧化活性。采用高效液相色谱-二极管阵列检测-电喷雾电离质谱联用技术对黄酮类化合物进行分析。共鉴定出11种化合物，其中6种，包括异鼠李素-3-O-二己糖苷、槲皮素-3-O-葡萄糖苷、槲皮素甲基醚-O-己糖苷、槲皮素、香叶木素和异鼠李素，首次在该植物中被描述。乙醇提取物的黄酮含量最高，其次是煎剂和浸剂（分别为25.4±0.8mg/g干物质、8.4±0.5mg/g干物质、0.2mg/g干物质），而煎剂表现出最佳的抗氧化活性（还原力的RC0.5=178μg/mL，脂质过氧化抑制率为72.4%，DPPH自由基清除活性的IC50=27.38μg/mL，NO自由基抑制率为59.7%）。这些结果表明，银色指甲草煎剂可被视为黄酮类化合物和抗氧化剂的宝贵来源，这可能有助于提升该植物的植物治疗潜力。
