Abdelkhalek Ahmed, Al-Askar Abdulaziz A, Alsubaie Maha M, Behiry Said I
Plant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab City, Alexandria 21934, Egypt.
Botany and Microbiology Department, Faculty of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Plants (Basel). 2021 Nov 11;10(11):2435. doi: 10.3390/plants10112435.
The widespread use of chemical control agents and pesticides for plant-pathogen control has caused many human health and environmental issues. Plant extracts and biocontrol agents have robust antimicrobial activity against different plant pathogens. However, their antiviral activities are still being investigated. In the present study, the methanol extract of was characterized and evaluated for its protective activity against the tobacco mosaic virus (TMV) infection in tomato plants under greenhouse conditions at 21 days post-inoculation. The results showed that the foliar application of extract (10 µg/mL) enhanced tomato plant growth, resulting in significant increases in shoot and root parameters and total chlorophyll contents. Moreover, a significant reduction in TMV accumulation level in -treated plants of 77.88% compared to non-treated plants was reported. Furthermore, induction of systemic resistance with significant elevation in production of antioxidant enzymes (PPO, CAT, and SOD) and transcriptional levels of the pathogenesis-related proteins (PR-1 and PR-7) and polyphenolic genes (CHS and HQT) were also observed. Out of 16 detected compounds, HPLC analysis revealed that the most abundant polyphenolic compounds found in extract were gallic acid (5.36 µg/mL), kaempferol (7.39 µg/mL), quercetin (7.44 µg/mL), ellagic acid (7.89 µg/mL), myricetin (8.36 µg/mL), and ferulic acid (8.69 µg/mL). The findings suggest that the use of extract as an effective and safe source for the production of bioactive compounds may offer a solution for a promising approach for the management of plant viral infections. To the best of our knowledge, this is the first report of the protective activity of extract against plant viral diseases.
化学防治剂和杀虫剂在植物病原体防治中的广泛使用引发了诸多人类健康和环境问题。植物提取物和生物防治剂对不同植物病原体具有强大的抗菌活性。然而，它们的抗病毒活性仍在研究之中。在本研究中，对[提取物名称未给出]的甲醇提取物进行了表征，并评估了其在温室条件下接种21天后对番茄植株烟草花叶病毒（TMV）感染的保护活性。结果表明，叶面喷施[提取物名称未给出]提取物（10 µg/mL）促进了番茄植株生长，导致地上部和根部参数以及总叶绿素含量显著增加。此外，据报道，与未处理植株相比，[提取物名称未给出]处理植株中的TMV积累水平显著降低了77.88%。此外，还观察到诱导了系统抗性，抗氧化酶（PPO、CAT和SOD）的产生以及病程相关蛋白（PR - 1和PR - 7）和多酚基因（CHS和HQT）的转录水平显著升高。在16种检测到的化合物中，HPLC分析显示，[提取物名称未给出]提取物中最丰富的多酚化合物是没食子酸（5.36 µg/mL）、山奈酚（7.39 µg/mL）、槲皮素（7.44 µg/mL）、鞣花酸（7.89 µg/mL）、杨梅素（8.36 µg/mL）和阿魏酸（8.69 µg/mL）。研究结果表明，使用[提取物名称未给出]提取物作为生产生物活性化合物的有效且安全的来源，可能为植物病毒感染管理的一种有前景的方法提供解决方案。据我们所知，这是[提取物名称未给出]提取物对植物病毒病保护活性的首次报道。
