Katanić Stanković Jelena S, Đorović Jovanović Jelena, Mišić Danijela, Gašić Uroš, Nikles Stefanie, Marković Zoran, Bauer Rudolf
Department of Science, Institute for Information Technologies Kragujevac, University of Kragujevac, Jovana Cvijića bb, 34000 Kragujevac, Serbia.
Department of Plant Physiology, Institute for Biological Research "Siniša Stanković"-National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
Molecules. 2023 Jan 28;28(3):1274. doi: 10.3390/molecules28031274.
(L.) M. T. Sharples & E. A. Tripp (Greater Stitchwort), formerly known as L., is widespread in the warm temperate areas of Europe and Western Asia, the Caucasus region, as well as in some countries of North Africa. Nowadays it is considered as a weed, but earlier it was often used raw in salads or for the treatment of various inflammatory disorders. The goal of this study was to determine the constituents of the methanol extract of aerial parts and its biological potential in terms of antioxidant, antimicrobial, and anti-inflammatory properties. Until now, the constituents and biological activities of this plant were not reported in detail. A comprehensive phytochemical profiling of the extract has shown that phenolic acids, such as ferulic, chlorogenic, and -coumaric acid, flavonoids and flavonoid glucosides, such as chrysoeriol, rutin, and naringin, are the most abundant compounds. The antioxidant activity of extract towards DPPH and ABTS radicals, but also the total antioxidant capacity and the inhibition of lipid peroxidation were moderate. The antimicrobial potential was pronounced mostly towards some fungi such as (MIC 1.25 mg/mL), whereas the capacity of to affect the growth of bacteria was much less pronounced. extract was most inclined to anti-inflammatory activity. At a concentration of 50 µg/mL, it significantly inhibited both cyclooxygenase enzymes (COX-1 and COX-2) by 71.24% and 72.83%, respectively. Molecular docking studies indicated that chlorogenic acid and chrysoeriol are the main contributors to COX-1 and COX-2 inhibitory activity.
（L.）M. T. 夏普尔斯和E. A. 特里普（大繁缕），以前称为L.，广泛分布于欧洲和西亚的暖温带地区、高加索地区以及北非的一些国家。如今它被视为一种杂草，但早些时候它常被生食用于沙拉或治疗各种炎症性疾病。本研究的目的是确定地上部分甲醇提取物的成分及其在抗氧化、抗菌和抗炎特性方面的生物潜力。到目前为止，这种植物的成分和生物活性尚未详细报道。提取物的全面植物化学分析表明，酚酸，如阿魏酸、绿原酸和对香豆酸，黄酮类化合物和黄酮苷，如芹菜素醇、芦丁和柚皮苷，是最丰富的化合物。提取物对DPPH和ABTS自由基的抗氧化活性，以及总抗氧化能力和脂质过氧化抑制作用均为中等。抗菌潜力主要表现在对一些真菌，如（最低抑菌浓度为1.25毫克/毫升），而其对细菌生长的影响能力则不太明显。提取物最倾向于抗炎活性。在浓度为50微克/毫升时，它分别显著抑制环氧化酶（COX - 1和COX - 2）71.24%和72.83%。分子对接研究表明，绿原酸和芹菜素醇是COX - 1和COX - 2抑制活性的主要贡献者。
