Rizal Govinda, Karki Shanta, Garcia Richard, Larazo Nikki, Alcasid Michael, Quick William Paul
C4 Rice Center, International Rice Research Institute (IRRI), Los Baños, Laguna, the Philippines.
C4 Rice Center, International Rice Research Institute (IRRI), Los Baños, Laguna, the Philippines; University of Sheffield, Sheffield, United Kingdom.
PLoS One. 2015 Apr 24;10(4):e0125092. doi: 10.1371/journal.pone.0125092. eCollection 2015.
An efficient method for crossing green foxtail (Setaria viridis) is currently lacking. S. viridis is considered to be the new model plant for the study of C4 system in monocots and so an effective crossing protocol is urgently needed. S. viridis is a small grass with C4-NADP (ME) type of photosynthesis and has the advantage of having small genome of about 515 Mb, small plant stature, short life cycle, multiple tillers, and profuse seed set, and hence is an ideal model species for research. The objectives of this project were to develop efficient methods of emasculation and pollination, and to speed up generation advancement. We assessed the response of S. viridis flowers to hot water treatment (48°C) and to different concentrations of gibberellic acid, abscisic acid, maleic hydrazide (MH), and kinetin. We found that 500 μM of MH was effective in the emasculation of S. viridis, whilst still retaining the receptivity of the stigma to pollination. We also report effective ways to accelerate the breeding cycle of S. viridis for research through the germination of mature as well as immature seeds in optimized culture media. We believe these findings will be of great interest to researchers using Setaria.
目前缺乏一种有效的绿色狗尾草（Setaria viridis）杂交方法。绿色狗尾草被认为是单子叶植物中研究C4系统的新型模式植物，因此迫切需要一种有效的杂交方案。绿色狗尾草是一种具有C4-NADP（ME）型光合作用的小草本植物，具有基因组小（约515 Mb）、植株矮小、生命周期短、分蘖多和结实量大等优点，因此是一种理想的研究模式物种。本项目的目标是开发有效的去雄和授粉方法，并加快世代进程。我们评估了绿色狗尾草花朵对热水处理（48°C）以及不同浓度赤霉素、脱落酸、马来酰肼（MH）和激动素的反应。我们发现500 μM的MH对绿色狗尾草去雄有效，同时仍能保持柱头对授粉的接受能力。我们还报告了通过在优化培养基中使成熟种子和未成熟种子发芽来加速绿色狗尾草研究育种周期的有效方法。我们相信这些发现将引起使用狗尾草的研究人员的极大兴趣。
