• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

跨膜脂质相互作用介导脂质锚定蛋白的纳米簇集。

Transbilayer lipid interactions mediate nanoclustering of lipid-anchored proteins.

作者信息

Raghupathy Riya, Anilkumar Anupama Ambika, Polley Anirban, Singh Parvinder Pal, Yadav Mahipal, Johnson Charles, Suryawanshi Sharad, Saikam Varma, Sawant Sanghapal D, Panda Aniruddha, Guo Zhongwu, Vishwakarma Ram A, Rao Madan, Mayor Satyajit

机构信息

National Centre for Biological Sciences (TIFR), Bellary Road, Bangalore 560 065, India; Shanmugha Arts, Science, Technology & Research Academy, Thanjavur 613401, India.

Raman Research Institute, C.V. Raman Avenue, Bangalore 560 080, India; Tampere University of Technology, Korkeakoulunkatu 10, 33720 Tampere, Finland.

出版信息

Cell. 2015 Apr 23;161(3):581-594. doi: 10.1016/j.cell.2015.03.048.

DOI:10.1016/j.cell.2015.03.048
PMID:25910209
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4651428/
Abstract

Understanding how functional lipid domains in live cell membranes are generated has posed a challenge. Here, we show that transbilayer interactions are necessary for the generation of cholesterol-dependent nanoclusters of GPI-anchored proteins mediated by membrane-adjacent dynamic actin filaments. We find that long saturated acyl-chains are required for forming GPI-anchor nanoclusters. Simultaneously, at the inner leaflet, long acyl-chain-containing phosphatidylserine (PS) is necessary for transbilayer coupling. All-atom molecular dynamics simulations of asymmetric multicomponent-membrane bilayers in a mixed phase provide evidence that immobilization of long saturated acyl-chain lipids at either leaflet stabilizes cholesterol-dependent transbilayer interactions forming local domains with characteristics similar to a liquid-ordered (lo) phase. This is verified by experiments wherein immobilization of long acyl-chain lipids at one leaflet effects transbilayer interactions of corresponding lipids at the opposite leaflet. This suggests a general mechanism for the generation and stabilization of nanoscale cholesterol-dependent and actin-mediated lipid clusters in live cell membranes.

摘要

理解活细胞膜中功能性脂质结构域是如何形成的一直是一项挑战。在此，我们表明跨膜相互作用对于由膜相邻动态肌动蛋白丝介导的糖基磷脂酰肌醇（GPI）锚定蛋白的胆固醇依赖性纳米簇的形成是必要的。我们发现形成GPI锚纳米簇需要长饱和酰基链。同时，在内侧小叶，含长酰基链的磷脂酰丝氨酸（PS）对于跨膜偶联是必需的。混合相中不对称多组分膜双层的全原子分子动力学模拟提供了证据，即在任一小叶固定长饱和酰基链脂质可稳定胆固醇依赖性跨膜相互作用，形成具有类似于液晶有序（lo）相特征的局部结构域。这通过实验得到验证，即在一个小叶固定长酰基链脂质会影响相对小叶上相应脂质的跨膜相互作用。这表明了活细胞膜中纳米级胆固醇依赖性和肌动蛋白介导的脂质簇生成和稳定的一般机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/bf3ef8c4db0b/nihms-737370-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/15624918d320/nihms-737370-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/2471afe5d244/nihms-737370-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/92ad61cbc693/nihms-737370-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/dd4d9a9d3eac/nihms-737370-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/b5f4af8f9387/nihms-737370-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/e64348ce8b3b/nihms-737370-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/bf3ef8c4db0b/nihms-737370-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/15624918d320/nihms-737370-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/2471afe5d244/nihms-737370-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/92ad61cbc693/nihms-737370-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/dd4d9a9d3eac/nihms-737370-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/b5f4af8f9387/nihms-737370-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/e64348ce8b3b/nihms-737370-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/282f/4651428/bf3ef8c4db0b/nihms-737370-f0007.jpg

相似文献

1
Transbilayer lipid interactions mediate nanoclustering of lipid-anchored proteins.跨膜脂质相互作用介导脂质锚定蛋白的纳米簇集。
Cell. 2015 Apr 23;161(3):581-594. doi: 10.1016/j.cell.2015.03.048.
2
Active emulsions in living cell membranes driven by contractile stresses and transbilayer coupling.受收缩应力和跨膜耦联驱动的活细胞膜中的活性乳液。
Proc Natl Acad Sci U S A. 2022 Jul 26;119(30):e2123056119. doi: 10.1073/pnas.2123056119. Epub 2022 Jul 22.
3
Sphingomyelin chain length influences the distribution of GPI-anchored proteins in rafts in supported lipid bilayers.鞘磷脂链长度影响糖基磷脂酰肌醇锚定蛋白在支持脂质双分子层中脂筏的分布。
Mol Membr Biol. 2007 May-Jun;24(3):233-42. doi: 10.1080/09687860601127770.
4
Transbilayer effects of raft-like lipid domains in asymmetric planar bilayers measured by single molecule tracking.通过单分子追踪测量不对称平面双层膜中类筏脂域的跨双层效应。
Biophys J. 2006 Nov 1;91(9):3313-26. doi: 10.1529/biophysj.106.091421. Epub 2006 Aug 11.
5
Assessment of the roles of ordered lipid microdomains in post-endocytic trafficking of glycosyl-phosphatidylinositol-anchored proteins in mammalian fibroblasts.评估有序脂质微区在糖基磷脂酰肌醇锚定蛋白在哺乳动物成纤维细胞中的内吞后运输中的作用。
Traffic. 2011 Aug;12(8):1012-24. doi: 10.1111/j.1600-0854.2011.01206.x. Epub 2011 Jun 22.
6
Interleaflet interaction and asymmetry in phase separated lipid bilayers: molecular dynamics simulations.层间相互作用和分相脂质双层的非对称性：分子动力学模拟。
J Am Chem Soc. 2011 May 4;133(17):6563-77. doi: 10.1021/ja106626r. Epub 2011 Apr 7.
7
Nanoclusters of GPI-anchored proteins are formed by cortical actin-driven activity.糖基磷脂酰肌醇锚定蛋白的纳米簇由皮质肌动蛋白驱动的活性形成。
Cell. 2008 Dec 12;135(6):1085-97. doi: 10.1016/j.cell.2008.11.032.
8
Bilayer registry in a multicomponent asymmetric membrane: dependence on lipid composition and chain length.多组分不对称膜中的双层排列：对脂质组成和链长的依赖性。
J Chem Phys. 2014 Aug 14;141(6):064903. doi: 10.1063/1.4892087.
9
Diffusion of GPI-anchored proteins is influenced by the activity of dynamic cortical actin.糖基磷脂酰肌醇（GPI）锚定蛋白的扩散受动态皮质肌动蛋白活性的影响。
Mol Biol Cell. 2015 Nov 5;26(22):4033-45. doi: 10.1091/mbc.E15-06-0397. Epub 2015 Sep 16.
10
Coupling of cholesterol-rich lipid phases in asymmetric bilayers.不对称双层膜中富含胆固醇的脂质相的耦合。
Biochemistry. 2008 Feb 19;47(7):2190-8. doi: 10.1021/bi7021552. Epub 2008 Jan 24.

引用本文的文献

1
The Vps13-like protein BLTP2 regulates phosphatidylethanolamine levels to maintain plasma membrane fluidity and breast cancer aggressiveness.Vps13样蛋白BLTP2调节磷脂酰乙醇胺水平以维持质膜流动性和乳腺癌侵袭性。
Nat Cell Biol. 2025 Jun 27. doi: 10.1038/s41556-025-01672-3.
2
The Bacterium Disperses Ordered Membrane Domains by Targeting Phase Boundaries.该细菌通过靶向相界来分散有序膜结构域。
Biomolecules. 2025 Feb 27;15(3):341. doi: 10.3390/biom15030341.
3
Sorting of complex sphingolipids within the cellular endomembrane systems.

本文引用的文献

1
Homo-FRET imaging highlights the nanoscale organization of cell surface molecules.同源荧光共振能量转移成像揭示了细胞表面分子的纳米级组织。
Methods Mol Biol. 2015;1251:151-73. doi: 10.1007/978-1-4939-2080-8_9.
2
Bilayer registry in a multicomponent asymmetric membrane: dependence on lipid composition and chain length.多组分不对称膜中的双层排列：对脂质组成和链长的依赖性。
J Chem Phys. 2014 Aug 14;141(6):064903. doi: 10.1063/1.4892087.
3
Caveolae regulate the nanoscale organization of the plasma membrane to remotely control Ras signaling.
细胞内膜系统中复杂鞘脂的分选
Front Cell Dev Biol. 2025 Feb 26;12:1490870. doi: 10.3389/fcell.2024.1490870. eCollection 2024.
4
Kingdom-specific lipid unsaturation calibrates sequence evolution in membrane arm subunits of eukaryotic respiratory complexes.特定王国的脂质不饱和度校准真核生物呼吸复合体膜臂亚基中的序列进化。
Nat Commun. 2025 Feb 27;16(1):2044. doi: 10.1038/s41467-025-57295-7.
5
Recent Advancements in Imaging Techniques for Individual Extracellular Vesicles.个体细胞外囊泡成像技术的最新进展
Molecules. 2024 Dec 10;29(24):5828. doi: 10.3390/molecules29245828.
6
The Effects of Viral Structural Proteins on Acidic Phospholipids in Host Membranes.病毒结构蛋白对宿主膜酸性磷脂的影响。
Viruses. 2024 Oct 31;16(11):1714. doi: 10.3390/v16111714.
7
Efficacy of Chitosan-N-Arginine Chitosomes in mRNA Delivery and Cell Viability Enhancement.壳聚糖-N-精氨酸壳聚糖体在mRNA递送及增强细胞活力方面的功效
ACS Appl Bio Mater. 2024 Dec 16;7(12):8261-8271. doi: 10.1021/acsabm.4c00983. Epub 2024 Nov 18.
8
Why nature evolved GPI-anchored proteins: unique structure characteristics enable versatile cell surface functions.为什么自然界会进化出糖基磷脂酰肌醇（GPI）锚定蛋白：独特的结构特征赋予其多样的细胞表面功能。
Glycobiology. 2024 Dec 10;34(12). doi: 10.1093/glycob/cwae089.
9
High-frequency MHz-order vibration enables cell membrane remodeling and lipid microdomain manipulation.高频兆赫兹级振动可实现细胞膜重塑和脂质微区调控。
Biophys J. 2025 Jan 7;124(1):25-39. doi: 10.1016/j.bpj.2024.10.007. Epub 2024 Oct 16.
10
Sphingolipids containing very long-chain fatty acids regulate Ypt7 function during the tethering stage of vacuole fusion.含有极长链脂肪酸的鞘脂在液泡融合的拴系阶段调节Ypt7功能。
J Biol Chem. 2024 Nov;300(11):107808. doi: 10.1016/j.jbc.2024.107808. Epub 2024 Sep 21.
小窝调节质膜的纳米级组织，从而远程控制 Ras 信号通路。
J Cell Biol. 2014 Mar 3;204(5):777-92. doi: 10.1083/jcb.201307055. Epub 2014 Feb 24.
4
Mutations in PGAP3 impair GPI-anchor maturation, causing a subtype of hyperphosphatasia with mental retardation.PGAP3 基因突变会损害 GPI-anchor 的成熟，导致伴有智力障碍的高磷酸血症亚型。
Am J Hum Genet. 2014 Feb 6;94(2):278-87. doi: 10.1016/j.ajhg.2013.12.012. Epub 2014 Jan 16.
5
Atomistic simulations of a multicomponent asymmetric lipid bilayer.多组分不对称脂质双层的原子级模拟。
J Phys Chem B. 2012 Nov 15;116(45):13403-10. doi: 10.1021/jp3032868. Epub 2012 Nov 1.
6
Active remodeling of cortical actin regulates spatiotemporal organization of cell surface molecules.皮质肌动蛋白的活性重塑调节细胞表面分子的时空组织。
Cell. 2012 Jun 8;149(6):1353-67. doi: 10.1016/j.cell.2012.05.008.
7
Myosin-1A targets to microvilli using multiple membrane binding motifs in the tail homology 1 (TH1) domain.肌球蛋白-1A 通过尾部同源 1（TH1）结构域中的多个膜结合基序靶向微绒毛。
J Biol Chem. 2012 Apr 13;287(16):13104-15. doi: 10.1074/jbc.M111.336313. Epub 2012 Feb 24.
8
Dynamic imaging of homo-FRET in live cells by fluorescence anisotropy microscopy.通过荧光各向异性显微镜对活细胞中的同源荧光共振能量转移进行动态成像。
Methods Enzymol. 2012;505:291-327. doi: 10.1016/B978-0-12-388448-0.00024-3.
9
Enhanced response of T lymphocytes from Pgap3 knockout mouse: Insight into roles of fatty acid remodeling of GPI anchored proteins.PGAP3 敲除小鼠 T 淋巴细胞反应增强：糖基磷脂酰肌醇锚定蛋白脂肪酸重塑作用的研究。
Biochem Biophys Res Commun. 2012 Jan 27;417(4):1235-41. doi: 10.1016/j.bbrc.2011.12.116. Epub 2011 Dec 29.
10
Probing protein heterogeneity in the plasma membrane using PALM and pair correlation analysis.使用 PALM 和对关联分析探测质膜中的蛋白质异质性。
Nat Methods. 2011 Sep 18;8(11):969-75. doi: 10.1038/nmeth.1704.