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[酶联免疫吸附测定法在人体包虫病免疫诊断中的应用。初步观察]

[The ELISA test in immunodiagnosis of human hydatidosis. Preliminary observations].

作者信息

Sorice F, Delia S, Castagnari L

出版信息

Boll Ist Sieroter Milan. 1977 Mar 31;56(2):152-6.

PMID:326274
Abstract

An evaluation of the E.L.I.S.A. method, compared to the indirect hemagglutination (I.H.) and to the counterimmunoelectrophoresis (CIEP) tests for the biological diagnosis of hydatidosis, was performed on 31 sera of patients, carrier of hydatid cysts, on 17 sera of patients with other infectious and parasitic diseases, and on 7 sera of blood donors. The immunoenzymatic test turned out positive in 30 out of the 31 sera of the patients with active hydatidosis (96.7%), while IH and CIEP turned out positive in 29 cases (93.5%). The control sera from patients with other diseases proved to be all negative, except one from a patient with active schistosomiasis. E.L.I.S.A. test was constantly negative with the sera of normal subjects. The validity and usefulness of E.L.I.S.A test for the diagnosis of the hydatid disease is discussed.

摘要

对31份患有包虫囊肿患者的血清、17份患有其他感染性和寄生虫病患者的血清以及7份献血者的血清进行了评估,以比较酶联免疫吸附测定(ELISA)法与间接血凝试验(IH)和对流免疫电泳(CIEP)试验在包虫病生物学诊断中的效果。在31份活动性包虫病患者的血清中,免疫酶试验有30份呈阳性(96.7%),而间接血凝试验和对流免疫电泳试验有29份呈阳性(93.5%)。除1份来自活动性血吸虫病患者的血清外,其他疾病患者的对照血清均为阴性。ELISA试验对正常受试者的血清始终呈阴性。本文讨论了ELISA试验在包虫病诊断中的有效性和实用性。

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