Department of Chemistry, Tamkang University, Tamsui 25137, Taiwan.
Department of Chemistry, Tamkang University, Tamsui 25137, Taiwan.
Anal Chim Acta. 2015 May 18;874:33-9. doi: 10.1016/j.aca.2015.02.035. Epub 2015 Feb 12.
In this approach, a novel method to fabricate an integrated amperometric platform used in off-channel electrophoresis has been introduced. A simple screen printed protocol combining a wet etching procedure was used to define the pattern on a glass substrate, and whole electrodes were constructed by filling the conductive carbon ink into the etched cavities. A simple Teflon tape was used to align this platform with the micro-channel, and the variation of reassembling of this device can be down to 2.2% without the assistance of microscope. This device was characterized by dopamine (DA) and catechol (CA), and the width of half peak is around 4s, even a 100 μm double T shape injection design and a 550 μm working electrode were used in this work. Under the optimum condition, this device possesses a low background with a noise level of 1.4 pA (peak to peak). The linear range for DA and CA are 0.1-100 μM (R = 0.998) and 0.2-200 μM (R = 0.996) with a theoretical plate number of 1.57 × 10(4) and 3.46 × 10(4) (plate/m), respectively.
在这种方法中，引入了一种新的方法来制造用于离通道电泳的集成安培平台。采用简单的丝网印刷协议结合湿法刻蚀工艺，在玻璃基板上定义图案，并通过将导电碳墨填充到蚀刻腔中来构建整个电极。使用简单的特氟隆胶带将该平台与微通道对准，并且在没有显微镜帮助的情况下，重新组装该装置的变化可以低至 2.2%。该装置通过多巴胺 (DA) 和儿茶酚 (CA) 进行了表征，半峰宽约为 4s，即使在这项工作中使用了 100μm 双 T 形注入设计和 550μm 工作电极。在最佳条件下，该装置具有低背景噪声水平为 1.4pA（峰峰值）。DA 和 CA 的线性范围分别为 0.1-100μM（R=0.998）和 0.2-200μM（R=0.996），理论板数分别为 1.57×10(4)和 3.46×10(4)（板/m）。
