Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, KS, USA.
Electrophoresis. 2013 Jul;34(14):2101-6. doi: 10.1002/elps.201300055.
Traditional fabrication methods for polymer microchips, the bonding of two substrates together to form the microchip, can make the integration of carbon electrodes difficult. We have developed a simple and inexpensive method to integrate graphite/PMMA composite electrodes (GPCEs) into a PMMA substrate. These substrates can be bonded to other PMMA layers using a solvent-assisted thermal bonding method. The optimal composition of the GPCEs for electrochemical detection was determined using cyclic voltammetry with dopamine as a test analyte. Using the optimized GPCEs in an all-PMMA flow cell with flow injection analysis, it was possible to detect 50 nM dopamine under the best conditions. These electrodes were also evaluated for the detection of dopamine and catechol following separation by MCE.
传统的聚合物微芯片制造方法，即将两个基底粘合在一起以形成微芯片，这使得碳电极的集成变得困难。我们开发了一种简单且廉价的方法，将石墨/PMMA 复合材料电极 (GPCE) 集成到 PMMA 基底上。这些基底可以使用溶剂辅助热键合方法与其他 PMMA 层结合。使用循环伏安法以多巴胺作为测试分析物，确定了用于电化学检测的 GPCE 的最佳组成。在具有流动注射分析的全 PMMA 流动池，使用优化的 GPCE 可以在最佳条件下检测到 50 nM 多巴胺。还通过微流控芯片电泳 (MCE) 分离后评估了这些电极对多巴胺和儿茶酚的检测。
