Guedes-Martins L, Silva E, Gaio A R, Saraiva J, Soares A I, Afonso J, Macedo F, Almeida H
Department of Experimental Biology, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal; IBMC-Instituto de Biologia Molecular e Celular, 4150-180 Porto, Portugal; Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-319 Porto, Portugal; Centro Hospitalar do Porto EPE, Departamento da Mulher e da Medicina Reprodutiva, Centro Hospitalar do Porto EPE, Largo Prof. Abel Salazar, 4099-001 Porto, Portugal.
Department of Experimental Biology, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal; IBMC-Instituto de Biologia Molecular e Celular, 4150-180 Porto, Portugal; Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-319 Porto, Portugal.
Redox Biol. 2015 Aug;5:114-123. doi: 10.1016/j.redox.2015.04.007. Epub 2015 Apr 20.
Blood flow assessment employing Doppler techniques is a useful procedure in pregnancy evaluation, as it may predict pregnancy disorders coursing with increased uterine vascular impedance, as pre-eclampsia. While the local causes are unknown, emphasis has been put on reactive oxygen species (ROS) excessive production. As NADPH oxidase (NOX) is a ROS generator, it is hypothesized that combining Doppler assessment with NOX activity might provide useful knowledge on placental bed disorders underlying mechanisms. A prospective longitudinal study was performed in 19 normal course, singleton pregnancies. Fetal aortic isthmus (AoI) and maternal uterine arteries (UtA) pulsatility index (PI) were recorded at two time points: 20-22 and 40-41 weeks, just before elective Cesarean section. In addition, placenta and placental bed biopsies were performed immediately after fetal extraction. NOX activity was evaluated using a dihydroethidium-based fluorescence method and associations to PI values were studied with Spearman correlations. A clustering of pregnancies coursing with higher and lower PI values was shown, which correlated strongly with placental bed NOX activity, but less consistently with placental tissue. The study provides evidence favoring that placental bed NOX activity parallels UtA PI enhancement and suggests that an excess in oxidation underlies the development of pregnancy disorders coursing with enhanced UtA impedance.
采用多普勒技术进行血流评估是孕期评估中的一项有用程序，因为它可以预测伴有子宫血管阻抗增加的妊娠疾病，如先兆子痫。虽然局部病因尚不清楚，但人们已将重点放在活性氧（ROS）的过度产生上。由于NADPH氧化酶（NOX）是一种ROS生成器，因此推测将多普勒评估与NOX活性相结合可能会为胎盘床疾病的潜在机制提供有用的信息。对19例正常单胎妊娠进行了一项前瞻性纵向研究。在两个时间点记录胎儿主动脉峡部（AoI）和母体子宫动脉（UtA）的搏动指数（PI）：在择期剖宫产术前的20 - 22周和40 - 41周。此外，胎儿娩出后立即进行胎盘和胎盘床活检。使用基于二氢乙锭的荧光方法评估NOX活性，并通过Spearman相关性研究其与PI值的关联。结果显示，PI值较高和较低的妊娠存在聚类现象，这与胎盘床NOX活性密切相关，但与胎盘组织的相关性不太一致。该研究提供的证据支持胎盘床NOX活性与UtA PI升高平行，并表明氧化过量是伴有UtA阻抗增加的妊娠疾病发展的基础。
