采用液相色谱-二极管阵列检测/质谱联用（LC-DAD/MS）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）技术对红葡萄酒葡萄皮渣中的花色苷和花色苷衍生色素进行筛选。

Screening of Anthocyanins and Anthocyanin-Derived Pigments in Red Wine Grape Pomace Using LC-DAD/MS and MALDI-TOF Techniques.

作者信息

Oliveira Joana, Alhinho da Silva Mara, Teixeira Natércia, De Freitas Victor, Salas Erika

机构信息

REQUIMTE - Laboratório Associado para a Quı́mica Verde, Departamento de Quı́mica e Bioquı́mica, Faculdade de Ciências, Universidade do Porto , Rua do Campo Alegre 687, 4169-007 Porto, Portugal.

Facultad de Ciencias Quı́micas, Universidad Autónoma de Chihuahua , Ciudad Universitaria s/n, C.P. 31170 Chihuahua, Mexico.

出版信息

J Agric Food Chem. 2015 Sep 9;63(35):7636-44. doi: 10.1021/acs.jafc.5b00256. Epub 2015 May 13.

DOI:10.1021/acs.jafc.5b00256

PMID:25912410

Abstract

Two phenolic extracts were made from a red wine grape pomace (GP) and fractionated first by sequential liquid-liquid extraction with organic solvents. The aqueous fraction was fractionated by low-pressure chromatography on Toyopearl HW-40 gel and on C18. Different fractions were obtained by sequential elution with aqueous/organic solvents, and then analyzed by liquid chromatography and mass spectrometry (LC-DAD/MS and MALDI-TOF). Over 50 anthocyanin-based pigments were detected by LC-DAD/MS in GP, mainly pyranoanthocyanins including A- and B-type vitisins and methylpyranoanthocyanins. The presence of oligomeric malvidin-3-O-coumaroylglucoside-based anthocyanins was also detected in GP using both LC-DAD/MS and MALDI-TOF.

摘要

从红酒葡萄皮渣（GP）中制备了两种酚类提取物，并首先通过用有机溶剂进行连续液-液萃取进行分离。水相部分通过在Toyopearl HW-40凝胶和C18上进行低压色谱法进行分离。通过用水/有机溶剂连续洗脱获得不同的馏分，然后通过液相色谱和质谱（LC-DAD/MS和MALDI-TOF）进行分析。通过LC-DAD/MS在GP中检测到50多种基于花青素的色素，主要是吡喃花青素，包括A型和B型葡萄素以及甲基吡喃花青素。使用LC-DAD/MS和MALDI-TOF在GP中也检测到了基于低聚锦葵色素-3-O-香豆酰葡萄糖苷的花青素的存在。