Njeruh F M, Gathuma J M, Tumboh-Oeri A G, Okelo G B
East Afr Med J. 1989 Mar;66(3):219-30.
Analysis for the amino acid composition of "Antigen 880" was carried out by use of double dimension paper chromatography and Biotronik 2,000 automatic amino acid analyzer. By the double dimension paper chromatography, leucine, phenylalanine, tyrosine and alanine were identified as amino acid components of the protein moiety of "Antigen 880". In the Biotronik 2,000 automatic amino acid analyzer showed the concentration of the various amino acids to be as follows: isoleucine, leucine, tyrosine, phenylalanine, lysine and histidine were identified as amino acid constituents of "Antigen 880". Quantitative studies in Biotronik 2,000 analyzer showed the concentration of the various amino acids to be as follows: valine -0.85 mumol/ml; leucine - 0.22 mumol/ml. /ml; iso-leucine - 0.18 mumol/ml; tyrosine - 0.04 mumol/ml, and histidine - 0.02 mumol/ml. The fatty acid composition of the lipid moiety of "Antigen 880" was investigated by use of Gas-Liquid chromatography. In this method, C8:0. C10:0, C12:0, C14:0, C16:0 and C18 were identified as the fatty acid constituents of the lipid moiety of "Antigen 880".
利用双向纸色谱法和百得灵2000型自动氨基酸分析仪对“抗原880”的氨基酸组成进行了分析。通过双向纸色谱法，鉴定出亮氨酸、苯丙氨酸、酪氨酸和丙氨酸是“抗原880”蛋白质部分的氨基酸成分。在百得灵2000型自动氨基酸分析仪上显示，各种氨基酸的浓度如下：异亮氨酸、亮氨酸、酪氨酸、苯丙氨酸、赖氨酸和组氨酸被鉴定为“抗原880”的氨基酸成分。在百得灵2000型分析仪上进行的定量研究显示，各种氨基酸的浓度如下：缬氨酸-0.85微摩尔/毫升；亮氨酸-0.22微摩尔/毫升；异亮氨酸-0.18微摩尔/毫升；酪氨酸-0.04微摩尔/毫升，组氨酸-0.02微摩尔/毫升。利用气液色谱法研究了“抗原880”脂质部分的脂肪酸组成。在该方法中，C8:0、C10:0、C12:0、C14:0、C16:0和C18被鉴定为“抗原880”脂质部分的脂肪酸成分。
