基于 DNA-QDs 树状大分子超结构的扩增电化学策略用于检测胸腺嘧啶 DNA 糖基化酶活性。
An amplified electrochemical strategy using DNA-QDs dendrimer superstructure for the detection of thymine DNA glycosylase activity.
机构信息
State Key Lab of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China; College of Life Information Science & Instrument Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China.
出版信息
Biosens Bioelectron. 2015 Sep 15;71:249-255. doi: 10.1016/j.bios.2015.04.048. Epub 2015 Apr 20.
A triple-signal amplification strategy was proposed for highly sensitive and selective detection of thymine DNA glycosylase (TDG) by coupling a dendrimer-like DNA label with the electrochemical method and quantum dots (QDs) tagging. The DNA-QDs dendrimer-like superstructure was designed by DNA hybridization and covalent assembling. Benefiting from outstanding performance of the amplification strategy, this assay showed high sensitivity, extraordinary stability, and easy operation. The limit of detection could reach 0.00003 U µL(-1) with a splendid specificity. The TDG content in different concentration of HeLa cell was also determined. This assay opens a new horizon for both qualitative and quantitative detection of TDG, holding great promise for potential application in cancer cell research and clinical diagnostics.
提出了一种三重信号放大策略，通过将树状大分子样 DNA 标记与电化学方法和量子点 (QDs) 标记相结合，用于高度敏感和选择性地检测胸腺嘧啶 DNA 糖基化酶 (TDG)。通过 DNA 杂交和共价组装设计了 DNA-QDs 树状大分子样超结构。受益于该放大策略的出色性能，该测定法表现出高灵敏度、非凡的稳定性和易于操作。检测限可达到 0.00003 U µL(-1)，具有出色的特异性。还测定了不同浓度 HeLa 细胞中的 TDG 含量。该测定法为 TDG 的定性和定量检测开辟了新的视野，有望在癌细胞研究和临床诊断中得到广泛应用。
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