Pang Xuehui, Wang Lin, Ma Hongmin, Zhang Yong, Pan Jihong, Chen Yao, Du Bin, Wei Qin
Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Shandong Medicinal Biotechnology Centre, the Key Lab for Biotechnology Drugs of Ministry of Health, the Key Lab of Rare and Uncommon Disease, Jinan 250022, China.
Sci Rep. 2016 Jan 18;6:19202. doi: 10.1038/srep19202.
The over expression of thioredoxin domain-containing protein 5 (TXNDC5) can promote the growth of castration-resistant prostate cancer (CRPC). A novel highly sensitive photoelectrochemical (PEC) aptsensor was developed for the detection of TXNDC5 by using the nanohybrids (TiO2 NRs/NCQDs) of nitrogen-doped carbon quantum dots (NCQDs) and TiO2 nanorods as the photo-to-electron conversion medium. TiO2 NRs/NCQDs nanohybrids were prepared by controlling the experimental condition. TiO2 NRs were self-assembled to form the nanopores with good photocurrent conversion efficiency. NCQDs possessed carboxyl groups (-COOH) and amino groups (-NH2) in the preparation process. -COOH and -NH2 groups played important roles for anchoring the capture probes (5' primer and 3' primer) through covalent binding. The ultrasensitive and stable detection for TXNDC5 was achieved by the specific recognition between the capture probes and the targets. The fabricated aptsensor showed excellent performance with a wide linear range (0.5 fmol/L ∼ 10 nmol/L) and a low detection limit of 0.1 fmol/L. This kind of aptsensor would provide a potential application for TXNDC5.
含硫氧还蛋白结构域蛋白5（TXNDC5）的过表达可促进去势抵抗性前列腺癌（CRPC）的生长。通过使用氮掺杂碳量子点（NCQDs）与TiO₂纳米棒的纳米杂化物（TiO₂ NRs/NCQDs）作为光生电子转换介质，开发了一种新型高灵敏度光电化学（PEC）适配体传感器用于检测TXNDC5。通过控制实验条件制备了TiO₂ NRs/NCQDs纳米杂化物。TiO₂ NRs自组装形成具有良好光电流转换效率的纳米孔。在制备过程中，NCQDs具有羧基（-COOH）和氨基（-NH₂）。-COOH和-NH₂基团通过共价结合在锚定捕获探针（5'引物和3'引物）方面发挥重要作用。通过捕获探针与靶标的特异性识别实现了对TXNDC5的超灵敏和稳定检测。所制备的适配体传感器表现出优异的性能，线性范围宽（0.5 fmol/L～10 nmol/L），检测限低至0.1 fmol/L。这种适配体传感器将为TXNDC5提供潜在的应用。
