Schubert Jörg, Ziegler Angelika, Rabenstein Frank
Institute for Biosafety in Plant Biotechnology, Julius Kühn-Institute, Federal Research Institute for Cultivated Plants, Erwin-Baur-Straße 27, 06484, Quedlinburg, Germany,
Arch Virol. 2015 Jul;160(7):1761-6. doi: 10.1007/s00705-015-2422-2. Epub 2015 Apr 28.
Wheat streak mosaic virus is a serious threat in wheat-producing countries. In Germany, the virus was first recorded in 2013 near Hoym. The complete sequence of isolate Hoym was obtained and compared to all other known complete WSMV sequences, including newly collected and sequenced isolates from France and Austria. Phylogenetic analysis revealed that the European isolates group together with those from the Middle East to form a separate cluster characterized by a distinct putative P1 protease cleavage site. By means of quantitative reverse transcription polymerase chain reaction, it was shown that RNA of the USA type strain PV57 accumulated to higher levels in infected wheat cv. Alcedo than did RNA of isolate Hoym.
小麦线条花叶病毒对小麦生产国构成严重威胁。在德国，该病毒于2013年首次在霍伊姆附近被发现。获得了霍伊姆分离株的完整序列，并与所有其他已知的完整小麦线条花叶病毒序列进行了比较，包括新收集并测序的来自法国和奥地利的分离株。系统发育分析表明，欧洲分离株与中东分离株聚集在一起，形成一个单独的簇，其特征是具有一个独特的假定P1蛋白酶切割位点。通过定量逆转录聚合酶链反应表明，美国典型毒株PV57的RNA在感染的小麦品种阿尔塞多中积累的水平高于霍伊姆分离株的RNA。
