Zschiesche Wiebke, Barth Olaf, Daniel Katharina, Böhme Sandra, Rausche Juliane, Humbeck Klaus
Institute of Biology, Martin-Luther-University Halle-Wittenberg, Weinbergweg 10, 06120, Halle, Germany.
New Phytol. 2015 Sep;207(4):1084-96. doi: 10.1111/nph.13419. Epub 2015 Apr 24.
Biotic and abiotic stress responses of plants are linked to developmental programs. Proteins involved in different signaling pathways are the molecular basis of this concerted interplay. In our study, we show that Arabidopsis thaliana HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN3 (HIPP3; At5g60800) acts as an upstream regulator of stress- and development-related regulatory networks. Localization, metal-binding and stress-responsive gene expression of HIPP3 were analyzed via microscopy, protein and inductively coupled plasma (ICP)-MS analyses and quantitative real-time PCR. In addition, transcriptome and phenotype analyses of plants overexpressing HIPP3 were used to unravel its function. Our data show that HIPP3 is a nuclear, zinc-binding protein. It is repressed during drought stress and abscisic acid (ABA) treatment and, similar to other pathogen-related genes, is induced after infection with Pseudomonas syringae pv. tomato. HIPP3 overexpression affects the regulation of > 400 genes. Strikingly, most of these genes are involved in pathogen response, especially in the salicylate pathway. In addition, many genes of abiotic stress responses and seed and flower development are affected by HIPP3 overexpression. Plants overexpressing HIPP3 show delayed flowering. We conclude that HIPP3 acts via its bound zinc as an upstream regulator of the salicylate-dependent pathway of pathogen response and is also involved in abiotic stress responses and seed and flower development.
植物的生物和非生物胁迫反应与发育程序相关联。参与不同信号通路的蛋白质是这种协同相互作用的分子基础。在我们的研究中，我们表明拟南芥重金属相关异戊二烯化植物蛋白3（HIPP3；At5g60800）作为胁迫和发育相关调控网络的上游调节因子发挥作用。通过显微镜、蛋白质和电感耦合等离子体质谱（ICP）分析以及定量实时PCR对HIPP3的定位、金属结合和胁迫响应基因表达进行了分析。此外，利用过表达HIPP3的植物的转录组和表型分析来揭示其功能。我们的数据表明，HIPP3是一种核锌结合蛋白。它在干旱胁迫和脱落酸（ABA）处理期间受到抑制，并且与其他病原体相关基因类似，在感染丁香假单胞菌番茄致病变种后被诱导。HIPP3过表达影响400多个基因的调控。值得注意的是，这些基因中的大多数参与病原体反应，尤其是在水杨酸途径中。此外，许多非生物胁迫反应以及种子和花发育的基因受到HIPP3过表达的影响。过表达HIPP3的植物开花延迟。我们得出结论，HIPP3通过其结合的锌作为病原体反应中水杨酸依赖性途径的上游调节因子发挥作用，并且还参与非生物胁迫反应以及种子和花的发育。
