Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, Guizhou Province, China.
Institute of Pomology Science, Guizhou Academy of Agricultural Sciences, Guiyang, 550006, People's Republic of China.
Planta. 2024 Nov 6;260(6):134. doi: 10.1007/s00425-024-04567-z.
The HIPP proteins are involved in low-temperature stress, the growth of sweet cherry, and may be potential targets for genetic improvement. PavHIPP16 improved cold resistance in Arabidopsis. In response to abiotic stressors, the heavy metal-associated isoprenylated plant protein (HIPP) proteins play a crucial regulatory role. Although the function of HIPP has been identified in some plants, there have been fewer systematic studies conducted on sweet cherry (Prunus avium L.). Therefore, we performed a comprehensive analysis and expression profiling of PavHIPPs using bioinformatics, RT-PCR, and qRT-PCR techniques. Our findings revealed that 28 PavHIPP genes were unevenly distributed across eight chromosomes. We predicted nine motifs in PavHIPP proteins and observed similar gene structures among highly homologous proteins. The promoter sequences of PavHIPPs contained numerous regulatory elements associated with an adversity of stress. The expression levels of some members showed varying degrees of change under low-temperature treatment. These genes were differentially expressed during flower and fruit development. Arabidopsis overexpressing the PavHIPP16 (OE) gene showed significantly lower relative conductivity and malondialdehyde (MDA) content compared with the wild-type (WT) plants under cold environment. Conversely, peroxidase (POD) activity, superoxide dismutase (SOD) activity, and proline content were significantly higher in OE Arabidopsis than in WT plants. Overall, our results suggest that PavHIPP16 OE Arabidopsis thaliana exhibited enhanced adaptability compared to WT plants under cold conditions. This study provides a foundation for future investigations of the pathways regulating sweet cherry growth and development mediated by the HIPP genes.
HIPP 蛋白参与低温胁迫、甜樱桃的生长，并且可能是遗传改良的潜在目标。PavHIPP16 提高了拟南芥的抗寒性。在应对非生物胁迫因子时，重金属相关异戊烯基化植物蛋白（HIPP）发挥着至关重要的调控作用。尽管 HIPP 的功能在一些植物中已经得到了鉴定，但在甜樱桃（Prunus avium L.）中进行的系统研究较少。因此，我们使用生物信息学、RT-PCR 和 qRT-PCR 技术对 PavHIPPs 进行了全面分析和表达谱分析。我们的研究结果表明，28 个 PavHIPP 基因不均匀地分布在 8 条染色体上。我们预测了 PavHIPP 蛋白中的 9 个基序，并观察到高度同源蛋白中的基因结构相似。PavHIPPs 的启动子序列包含许多与逆境相关的调控元件。一些成员的表达水平在低温处理下表现出不同程度的变化。这些基因在花和果实发育过程中表现出不同程度的差异表达。与野生型（WT）相比，拟南芥中过表达 PavHIPP16（OE）基因的相对电导率和丙二醛（MDA）含量在冷环境下显著降低。相反，OE 拟南芥中的过氧化物酶（POD）活性、超氧化物歧化酶（SOD）活性和脯氨酸含量明显高于 WT 植物。总体而言，我们的结果表明，在低温条件下，PavHIPP16 OE 拟南芥比 WT 植物表现出更强的适应性。这项研究为进一步研究 HIPP 基因调控甜樱桃生长和发育的途径提供了基础。
