Brittain G P, Rostron C K, Morton D B, Rees J E
Department of Ophthalmology, Leicester Royal Infirmary.
Eye (Lond). 1989;3 ( Pt 1):56-63. doi: 10.1038/eye.1989.8.
An adhesive made of concentrated human fibrinogen produced from samples of single donor blood by a simple cryoprecipitation method was tested for its ability to bond lamellar corneal buttons in vitro and found to have a bond strength of 140 gm/cm2. The adhesive was successfully used to attach experimental epikeratophakia grafts in a rabbit model. The results were similar to those reported from an earlier trial using a commercially available adhesive prepared from pooled multi-donor human plasma. This experimental trial of single-donor adhesive demonstrates the possibility of successful use of autologous fibrinogen adhesive in human epikeratophakia. Autologous adhesive would remove the possible threat of transmitted disease posed by multi-donor adhesive and avoid immune reactions to foreign proteins.
通过简单的冷沉淀法从单供体血液样本中制备的浓缩人纤维蛋白原制成的粘合剂，在体外测试了其粘合层状角膜纽扣的能力，发现其粘合强度为140克/平方厘米。该粘合剂已成功用于兔模型中附着实验性表层角膜镜片术移植物。结果与早期使用由多供体人血浆制备的市售粘合剂的试验报告相似。这项单供体粘合剂的实验性试验证明了在人类表层角膜镜片术中成功使用自体纤维蛋白原粘合剂的可能性。自体粘合剂将消除多供体粘合剂带来的传播疾病的潜在威胁，并避免对外源蛋白质的免疫反应。
