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利用热带假丝酵母 NBRC 0618 发酵橄榄修剪物酸水解物生产乙醇和木糖醇。

Ethanol and xylitol production by fermentation of acid hydrolysate from olive pruning with Candida tropicalis NBRC 0618.

机构信息

Department of Chemical, Environmental and Material Engineering, University of Jaén, 23071 Jaén, Spain.

Department of Chemical, Environmental and Material Engineering, University of Jaén, 23071 Jaén, Spain.

出版信息

Bioresour Technol. 2015 Aug;190:1-6. doi: 10.1016/j.biortech.2015.04.045. Epub 2015 Apr 22.

DOI:10.1016/j.biortech.2015.04.045
PMID:25916261
Abstract

Olive tree pruning biomass has been pretreated with pressurized steam, hydrolysed with hydrochloric acid, conditioned and afterwards fermented using the non-traditional yeast Candida tropicalis NBRC 0618. The main aim of this study was to analyse the influence of acid concentration on the hydrolysis process and its effect on the subsequent fermentation to produce ethanol and xylitol. From the results, it could be deduced that both total sugars and d-glucose recovery were enhanced by increasing the acid concentration tested; almost the whole hemicellulose fraction was hydrolysed when 3.77% was used. It has been observed a sequential production first of ethanol, from d-glucose, and then xylitol from d-xylose. The overall ethanol and xylitol yields ranged from 0.27 to 0.38kgkg(-1), and 0.12 to 0.23kgkg(-1) respectively, reaching the highest values in the fermentation of the hydrolysates obtained with hydrochloric acid 2.61% and 1.11%, respectively.

摘要

橄榄油修剪生物质先用加压蒸汽预处理,再用盐酸水解,然后用非传统酵母热带假丝酵母 NBRC 0618 进行发酵。本研究的主要目的是分析酸浓度对水解过程的影响及其对随后生产乙醇和木糖醇的发酵的影响。结果表明,通过提高所测试的酸浓度,可以提高总糖和 d-葡萄糖的回收率;当使用 3.77%的酸时,几乎可以水解整个半纤维素部分。观察到首先从 d-葡萄糖生产乙醇,然后从 d-木糖生产木糖醇的顺序生产。总的乙醇和木糖醇产率分别在 0.27 到 0.38kgkg(-1)和 0.12 到 0.23kgkg(-1)之间变化,在使用盐酸 2.61%和 1.11%获得的水解产物的发酵中达到最高值。

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