Iskandar Maria Emil, Aslani Arash, Tian Qiaomu, Liu Huinan
Department of Bioengineering, University of California at Riverside, 900 University Avenue, MSE 227, Riverside, CA, 92521, USA.
J Mater Sci Mater Med. 2015 May;26(5):189. doi: 10.1007/s10856-015-5512-5. Epub 2015 Apr 28.
This article reports the deposition and characterization of nanostructured calcium phosphate (nCaP) on magnesium-yttrium alloy substrates and their cytocompatibility with bone marrow derived mesenchymal stem cells (BMSCs). The nCaP coatings were deposited on magnesium and magnesium-yttrium alloy substrates using proprietary transonic particle acceleration process for the dual purposes of modulating substrate degradation and BMSC adhesion. Surface morphology and feature size were analyzed using scanning electron microscopy and quantitative image analysis tools. Surface elemental compositions and phases were analyzed using energy dispersive X-ray spectroscopy and X-ray diffraction, respectively. The deposited nCaP coatings showed a homogeneous particulate surface with the dominant feature size of 200-500 nm in the long axis and 100-300 nm in the short axis, and a Ca/P atomic ratio of 1.5-1.6. Hydroxyapatite was the major phase identified in the nCaP coatings. The modulatory effects of nCaP coatings on the sample degradation and BMSC behaviors were dependent on the substrate composition and surface conditions. The direct culture of BMSCs in vitro indicated that multiple factors, including surface composition and topography, and the degradation-induced changes in media composition, influenced cell adhesion directly on the sample surface, and indirect adhesion surrounding the sample in the same culture. The alkaline pH, the indicator of Mg degradation, played a role in BMSC adhesion and morphology, but not the sole factor. Additional studies are necessary to elucidate BMSC responses to each contributing factor.
本文报道了纳米结构磷酸钙（nCaP）在镁钇合金基底上的沉积与表征及其与骨髓间充质干细胞（BMSC）的细胞相容性。采用专有的跨音速粒子加速工艺将nCaP涂层沉积在镁和镁钇合金基底上，以实现调节基底降解和BMSC黏附的双重目的。使用扫描电子显微镜和定量图像分析工具分析表面形态和特征尺寸。分别使用能量色散X射线光谱和X射线衍射分析表面元素组成和相。沉积的nCaP涂层呈现出均匀的颗粒表面，长轴上的主要特征尺寸为200 - 500 nm，短轴上为100 - 300 nm，Ca/P原子比为1.5 - 1.6。羟基磷灰石是在nCaP涂层中鉴定出的主要相。nCaP涂层对样品降解和BMSC行为的调节作用取决于基底组成和表面条件。BMSC的体外直接培养表明，多种因素，包括表面组成和形貌以及降解引起的培养基组成变化，直接影响细胞在样品表面的黏附，并间接影响同一培养物中样品周围的黏附。碱性pH值作为镁降解的指标，在BMSC黏附和形态方面发挥作用，但不是唯一因素。需要进一步研究以阐明BMSC对每个影响因素的反应。
