Zhu Xueping, Zhang Xiaomin, Yu Lingling, Xu Yumin, Feng Xing, Wang Jian
Department of Neonatology, Children's Hospital Affiliated to Soochow University, Suzhou, Jiangsu 215003, China.
Department of Neonatology Surgery, Children's Hospital Affiliated to Soochow University, Suzhou, Jiangsu 215003, China.
Gastroenterol Res Pract. 2015;2015:269831. doi: 10.1155/2015/269831. Epub 2015 Mar 30.
Objective. To use a rabbit model of parenteral nutrition-associated liver disease (PNALD) to study changes of the endoplasmic reticulum stress (ERS) marker glucose regulatory protein 94 (GRP94) and determine its role in the pathogenesis of PNALD. Methods. A rabbit PNALD model total parenteral nutrition (TPN) group was established. A corresponding control group received breast-feeding for one week. Serum biochemical parameters were measured and liver histological examinations were performed. The level of GRP94 mRNA and protein were measured. Results. The results showed that the serum TBIL, DBIL, and γ-GT levels in the TPN group were significantly higher than those in the control group, while levels of serum ALB in TPN group were significantly lower than those in the control group. The immunohistochemistry results showed that the protein expression level of GRP94 in the liver of TPN group was significantly increased compared with the control group. The RT-PCR results showed that the level of GRP94 mRNA in the liver of the TPN group was significantly higher compared with the control group. Conclusions. The mRNA and protein levels of GRP94 in the TPN group were both significantly increased, indicating that ERS may be directly related to the occurrence and development of PNALD.
目的。利用肠外营养相关肝病（PNALD）兔模型研究内质网应激（ERS）标志物葡萄糖调节蛋白94（GRP94）的变化，并确定其在PNALD发病机制中的作用。方法。建立兔PNALD模型全肠外营养（TPN）组。相应的对照组母乳喂养一周。检测血清生化参数并进行肝脏组织学检查。检测GRP94 mRNA和蛋白水平。结果。结果显示，TPN组血清总胆红素（TBIL）、直接胆红素（DBIL）和γ-谷氨酰转移酶（γ-GT）水平显著高于对照组，而TPN组血清白蛋白（ALB）水平显著低于对照组。免疫组化结果显示，TPN组肝脏中GRP94蛋白表达水平与对照组相比显著升高。逆转录-聚合酶链反应（RT-PCR）结果显示，TPN组肝脏中GRP94 mRNA水平与对照组相比显著升高。结论。TPN组GRP94的mRNA和蛋白水平均显著升高，表明ERS可能与PNALD的发生发展直接相关。
