Suppression of IRE1 Attenuated the Fatty Degeneration in Parenteral Nutrition-Related Liver Disease (PNALD) Cell Model.

AIMS

To model the parenteral nutrition-associated liver disease (PNALD) in rat normal hepatocytes BRL and investigate the role of endoplasmic reticulum stress- (ERS-) related IRE1 signal in the process of PNALD.

METHODS

The BRL cells were treated with different concentrations of soybean oil emulsion (SO) to induce hepatocyte fatty degeneration. The PNALD cell disease model was further confirmed by analysis of Oil Red O staining and biochemical parameters. Next, the IRE1 signal in the process of PNALD. signal in the process of PNALD. signal in the process of PNALD. signal in the process of PNALD.

RESULTS

The results of Oil Red O staining indicated that the PNALD was successfully established in BRL cells and the CCK-8 data indicated which 0.6% that SO was further applied to the experiment owing to its better induction of PNALD and less toxicity to the cells. Besides, the value of biochemical parameters (TBIL, DBIL, ALT, and AST) was also elevated in the SO group compared with the NG group. After knockdown of IRE1 signal in the process of PNALD. signal in the process of PNALD.

CONCLUSION

IRE1 was induced in PNALD cell model and suppression of IRE1 resulted in reduced steatosis in this cell disease model. Taken together, our data suggested that the IRE1 pathway may be involved in the development of PNALD. signal in the process of PNALD. signal in the process of PNALD. signal in the process of PNALD.