[A fast determination of serum bilirubin with the interference from hemolysis and turbidity by three wavelength spectrophotometry].

In determination of bilirubin in serum by means of color reaction when hemolysis and turbidity are present, the reliability of the measurement is decreased by the interference from hemoglobin and other turbidities. Direct measurement of bilirubin in serum by three wavelength spectrophotometry at 500 nm, 450 nm and 370 nm instead of by color reaction, provides a fast, semi-micro and accurate way without the use of any reagents. Besides the rise of the baseline by blood cells and turbidities can be corrected without the consideration of hemolysis. Under the given experimental condition, the mean recovery is 96.3 +/- 2.41% (n = 6), coefficient of variation (CV) is 4.0%.