一种使用铁氰化钾自动测量血清总胆红素的可靠方法。
A robust procedure for the automated measurement of total serum bilirubin using potassium ferricyanide.
作者信息
O'Leary N, Pembroke A, Duggan P F
机构信息
Biochemistry Laboratory, Regional Hospital, Cork, Ireland.
出版信息
Ann Clin Biochem. 1993 Mar;30 ( Pt 2):175-9. doi: 10.1177/000456329303000211.
Bilirubin is oxidized to biliverdin by 1.0 mmol/L potassium ferricyanide in the presence of caffeine. The absorbance decline at 480 nm with blanking at 660 nm is proportional to bilirubin concentration. The assay is linear up to 1000 mumol/L and is suitable for the measurement of bilirubin in adult and neonatal patients. The assay is accurate and precise and is not significantly affected by haemolysis up to 5.0 g/L haemoglobin. Reagents and calibration are stable for at least 3 months.
在咖啡因存在的情况下，胆红素被1.0毫摩尔/升铁氰化钾氧化为胆绿素。在660纳米处进行空白校正后，480纳米处的吸光度下降与胆红素浓度成正比。该测定法在高达1000微摩尔/升时呈线性，适用于成人和新生儿患者胆红素的测定。该测定法准确且精密，血红蛋白浓度高达5.0克/升时的溶血对其无显著影响。试剂和校准至少3个月稳定。
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