Kelly A, McKenna J P, McLelland A, Percy R A, Spooner R J
Clin Chem. 1979 Aug;25(8):1482-4.
We describe a method for the bichromatic determination of total bilirubin with a CentrifiChem 400 centrifugal analyzer with the Boehringer DPD kit. After reading the blank at 620 nm, we read the test at 550 nm. Because only one rotor is required, the analytical time for bilirubin estimations is halved. The proposed method is no more prone to interference by hemoglobin or turbidity than a conventional approach or a reference continuous-flow method and has performed well over several months of routine use.
我们描述了一种使用Boehringer DPD试剂盒，通过CentrifiChem 400离心分析仪对总胆红素进行双色测定的方法。在620nm处读取空白值后，于550nm处读取测试值。由于仅需一个转子，胆红素测定的分析时间减半。与传统方法或参考连续流动法相比，该方法不易受血红蛋白或浊度干扰，并且在数月的常规使用中表现良好。
