Crabb D W, Stein P M, Dipple K M, Hittle J B, Sidhu R, Qulali M, Zhang K, Edenberg H J
Department of Medicine, Indiana University School of Medicine, Indianapolis 46223.
Genomics. 1989 Nov;5(4):906-14. doi: 10.1016/0888-7543(89)90133-x.
Clones containing the rat class I alcohol dehydrogenase (ADH) gene were isolated from a Charon 4A genomic library. The gene spans approximately 13 kb and comprises nine exons and eight introns. The upstream 436 bp contain canonical TATA and CCAAT sequences, an inverted CACCC box, a TG3 box found in mouse and human ADH promoters, and regions of homology to glucocorticoid response elements. The 5'-untranslated region of the ADH transcript has the potential to form a stable stem-loop structure. The first intron contains an unusual stretch of alternating purines and pyrimidines similar to that found in the same location in the mouse ADH gene. The amino acid insertion found in rat alcohol dehydrogenase results from a shift in the 3' splice junction of the fourth intron which adds an extra three base pairs to the fifth exon. Intron-exon boundaries are otherwise identical to those in mouse and human ADH genes. H4IIE cells stably transfected with plasmids containing the chloramphenicol acetyltransferase (CAT) gene fused behind the first 436 bp of the promoter region express CAT, but the CAT activity is not inducible by dexamethasone. The elements responsible for glucocorticoid stimulation of ADH gene transcription appear to reside outside of this region.
从Charon 4A基因组文库中分离出含有大鼠I类乙醇脱氢酶（ADH）基因的克隆。该基因跨度约为13 kb，由9个外显子和8个内含子组成。上游436 bp包含典型的TATA和CCAAT序列、一个反向的CACCC框、在小鼠和人类ADH启动子中发现的TG3框以及与糖皮质激素反应元件的同源区域。ADH转录本的5'-非翻译区有形成稳定茎环结构的潜力。第一个内含子包含一段不寻常的嘌呤和嘧啶交替序列，类似于在小鼠ADH基因相同位置发现的序列。大鼠乙醇脱氢酶中发现的氨基酸插入是由于第四个内含子的3'剪接位点发生移位，使第五个外显子额外增加了三个碱基对。内含子-外显子边界在其他方面与小鼠和人类ADH基因相同。用含有在启动子区域前436 bp后融合氯霉素乙酰转移酶（CAT）基因的质粒稳定转染的H4IIE细胞表达CAT，但CAT活性不能被地塞米松诱导。负责糖皮质激素刺激ADH基因转录的元件似乎位于该区域之外。
