School of Environmental Engineering, Wuhan Textile University, Wuhan 430073, PR China.
School of Environmental Engineering, Wuhan Textile University, Wuhan 430073, PR China.
Bioresour Technol. 2015 Aug;190:36-43. doi: 10.1016/j.biortech.2015.04.037. Epub 2015 Apr 22.
A simplified kinetics model was built to study the inhibition of glucose on cellulosome of Clostridium thermocellum. Suitable reaction conditions were adopted to evaluate the model. The model was evaluated at different temperatures and further with various activated carbon additions as adsorbent for glucose. Investigation results revealed that the model could describe the hydrolysis kinetics of cellulose by cellulosome quite well. Glucose was found to be an inhibitor for cellulosome based on the kinetics analysis. Inhibition increased with the increase in temperature. Activated carbon as adsorbent could lower the inhibition. Parameters in the model were further discussed based on the experiment. The model might also be used to describe the strong inhibition of cellobiose on cellulosome. Saccharification of cellulose by both cellulosome and C. thermocellum could be enhanced efficiently by activated carbon addition.
建立简化动力学模型以研究葡萄糖对热纤梭菌细胞纤维素酶的抑制作用。采用合适的反应条件对模型进行评估。在不同温度下以及进一步添加不同量的活性炭作为葡萄糖吸附剂的条件下对模型进行评估。研究结果表明，该模型可以很好地描述细胞纤维素酶对纤维素的水解动力学。根据动力学分析，发现葡萄糖是细胞纤维素酶的抑制剂。随着温度的升高，抑制作用增强。活性炭作为吸附剂可以降低抑制作用。根据实验进一步讨论了模型中的参数。该模型也可用于描述纤维二糖对细胞纤维素酶的强烈抑制作用。通过添加活性炭，可以有效地提高细胞纤维素酶和热纤梭菌对纤维素的糖化作用。
