Tamer Gülden Sönmez, Dündar Devrim, Uzuner Hüseyin, Baydemir Canan
Department of Medical Microbiology, Kocaeli University, Faculty of Medicine, Kocaeli, Turkey.
Department of Medical Microbiology, Kocaeli University, Institute of Health Sciences, Kocaeli, Turkey.
Med Sci Monit. 2015 Apr 29;21:1219-22. doi: 10.12659/MSM.893155.
Echinococcosis in humans is a disease caused by the larvae of Echinococcus granulosus (E. granulosus) and Echinococcus multilocularis (E. multilocularis). Serological tests are valuable, especially in the clarification of unexplained clinical findings and imaging methods. For this reason, indirect hemagglutination (IHA), latex agglutination, immunoelectrophoresis, immunoblotting, immuno-enzymatic tests, indirect fluorescence antibody test (IFAT), and enzyme-linked immunosorbent assay (ELISA) are used. The purpose of this study was to investigate the value of an immunochromatographic test (ICT) specific for E. granulosus antibodies in the diagnosis of echinococcosis.
MATERIAL/METHODS: ICT evaluated 102 cases of cystic echinococcosis, 38 cases of other parasitic diseases, and 50 healthy individuals. ELISA (DRG, Germany) that detects IgG antibodies specific for E. granulosus was used as the reference method.
The sensitivity, specificity, and positive and negative predictive values of ICT were 96.8%, 87.5%, 98.9%, and 70%, respectively. Diagnostic value was 96.1%. No significant differences and high degrees of agreement were found between ELISA and immunochromatographic test for cystic echinococcosis. Serum samples included 4 taeniasis, 2 leishmaniasis, and 2 healthy individuals were diagnosed to be positive with immunochromatographic test.
The ability of test to give fast results without need for equipment, devices, and specific storage conditions is an advantage. This test may be used due to its advantages in endemic regions for screening and diagnostic purposes.
人类棘球蚴病是由细粒棘球绦虫（E. granulosus）和多房棘球绦虫（E. multilocularis）的幼虫引起的疾病。血清学检测很有价值，尤其是在明确不明原因的临床发现和影像学方法方面。因此，使用了间接血凝试验（IHA）、乳胶凝集试验、免疫电泳、免疫印迹、免疫酶试验、间接荧光抗体试验（IFAT）和酶联免疫吸附测定（ELISA）。本研究的目的是探讨一种针对细粒棘球绦虫抗体的免疫层析试验（ICT）在棘球蚴病诊断中的价值。
材料/方法：ICT评估了102例囊性棘球蚴病患者、38例其他寄生虫病患者和50名健康个体。使用检测细粒棘球绦虫特异性IgG抗体的ELISA（德国DRG公司）作为参考方法。
ICT的敏感性、特异性、阳性和阴性预测值分别为96.8%、87.5%、98.9%和70%。诊断价值为96.1%。ELISA和免疫层析试验在囊性棘球蚴病诊断方面未发现显著差异且一致性较高。血清样本中包括4例绦虫病、2例利什曼病患者以及2名健康个体经免疫层析试验诊断为阳性。
该试验无需设备、仪器和特定储存条件就能快速得出结果，这是一个优点。由于其优势，该试验可在流行地区用于筛查和诊断目的。
