Chanprasertyothin Suwannee, Jongjaroenprasert Wallaya, Ongphiphadhanakul Boonsong
Research Center, Ramathibodi Hospital, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand.
Department of Medicine, Ramathibodi Hospital, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand.
J Diabetes Res. 2015;2015:216383. doi: 10.1155/2015/216383. Epub 2015 Apr 2.
The aim of this study is to investigate the insulin-like growth factor type 2 (IGF2R) gene and circulating soluble IGF2R in relation to type 2 diabetes (T2DM). Six hundred fifty-four subjects without history of diabetes were screened for diabetes by oral glucose tolerance test. In addition, 145 subjects with known diabetes were recruited from a local diabetes clinic. Circulating IGF2R levels were measured by ELISA method; plasma glucose was measured by colorimetric method; insulin levels were determined by chemiluminescent method; IGF2R gene rs416572 was genotyped using real-time PCR. The distributions of IGF2R genotypes were 69.2% CC, 27.8% CT, and 3.0% TT. The C allele was more commonly found in diabetes subjects, with a significant difference (P < 0.01). In the presence of the T allele, circulating IGF2R levels were significantly lower (P < 0.05). There was no significant difference in other potential confounders including age, sex, and BMI. Only circulating IGF2R, age, and BMI were independently associated with the degree of insulin resistance, as assessed by the HOMA model. It was found that age, sex, and BMI were associated with beta cell function. In conclusion, IGF2R gene polymorphism and circulating IGF2R are associated with T2DM.
本研究旨在探讨2型胰岛素样生长因子（IGF2R）基因及循环可溶性IGF2R与2型糖尿病（T2DM）的关系。通过口服葡萄糖耐量试验对654名无糖尿病病史的受试者进行糖尿病筛查。此外，从当地糖尿病诊所招募了145名已知糖尿病患者。采用酶联免疫吸附测定（ELISA）法检测循环IGF2R水平；采用比色法检测血糖；采用化学发光法测定胰岛素水平；采用实时聚合酶链反应（PCR）对IGF2R基因rs416572进行基因分型。IGF2R基因型分布为CC型69.2%、CT型27.8%、TT型3.0%。C等位基因在糖尿病患者中更为常见，差异有统计学意义（P<0.01）。存在T等位基因时，循环IGF2R水平显著降低（P<0.05）。在年龄、性别和体重指数等其他潜在混杂因素方面无显著差异。根据稳态模型评估，只有循环IGF2R、年龄和体重指数与胰岛素抵抗程度独立相关。研究发现，年龄、性别和体重指数与β细胞功能有关。总之，IGF2R基因多态性和循环IGF2R与T2DM有关。
