Yan Zhengsong, Chen Dan, Shen Yiping, Ye Baodong
a Department of Hematology , Zhejiang Traditional Chinese Medical Hospital , Hangzhou , Zhejiang , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2671-2. doi: 10.3109/19401736.2015.1022752. Epub 2015 Apr 29.
Aspergillus flavus is a haploid filamentous fungus that is common in the environment and has been implicated in human infections. The complete mitochondrial genome of A. flavus has been determined by high-throughput sequencing technology in this work. Our study revealed that the mitochondrial genome of A. flavus is 31,602 bp long, with an A + T content of 74.83%, which consists of a usual set of mitochondrial proteins and RNA genes, including large and small ribosomal RNAs, 15 proteins, and 20 tRNA genes and contains two introns. Notably, it also contains two hypothetical proteins without obvious homology to any known proteins. All structural genes are located on one strand and are apparently transcribed in one direction. Codon usage analysis indicated that all protein coding genes employ the standard fungal mitochondrial start and stop codons; and the nucleotide bias toward AT was also reflected in the codon usage. The complete mitochondrial genomes of A. flavus would be useful for future investigation of the genetic, evolution, and clinical identification of Aspergillus species.
黄曲霉是一种单倍体丝状真菌，在环境中很常见，并与人类感染有关。在本研究中，通过高通量测序技术确定了黄曲霉的完整线粒体基因组。我们的研究表明，黄曲霉的线粒体基因组长度为31,602 bp，A+T含量为74.83%，由一组常见的线粒体蛋白质和RNA基因组成，包括大、小核糖体RNA、15种蛋白质和20种tRNA基因，并含有两个内含子。值得注意的是，它还包含两个与任何已知蛋白质无明显同源性的假设蛋白质。所有结构基因都位于一条链上，显然是按一个方向转录的。密码子使用分析表明，所有蛋白质编码基因都使用标准的真菌线粒体起始和终止密码子；并且密码子使用中也反映出对AT的核苷酸偏好。黄曲霉的完整线粒体基因组将有助于未来对曲霉菌种的遗传、进化和临床鉴定的研究。
