Ruan Zhi, Dai Fangwei, Fang Xu, Chen Huan, Yu Dongliang
a Biomedical Research Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University , Hangzhou , Zhejiang , China.
b Center of Experimental Animals, Zhejiang Academy of Medical Sciences , Hangzhou , Zhejiang , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):3876-3877. doi: 10.3109/19401736.2014.987241. Epub 2014 Dec 8.
Aspergillus ustus is among the most ubiquitous soil species and has been implicated in human infections. The complete mitochondrial genome of A. ustus has been determined by high-throughput sequencing technology in this work. Our study revealed that the mitochondrial genome of A. ustus is 33,007 bp long, with AT content of 74.84%, which consists of a usual set of mitochondrial proteins and RNA genes, including large and small ribosomal RNAs, 15 proteins and 20 tRNA genes and contains two introns. Notably, it also contains three hypothetical proteins without obvious homology to any known proteins. All structural genes are located on one strand and are apparently transcribed in one direction. The complete mitochondrial genomes of A. ustus would be useful for future investigation of the genetics, evolution and clinical identification of Aspergillus species.
黑曲霉是最常见的土壤物种之一，并与人类感染有关。在本研究中，通过高通量测序技术测定了黑曲霉的完整线粒体基因组。我们的研究表明，黑曲霉的线粒体基因组长度为33,007 bp，AT含量为74.84%，由一组常见的线粒体蛋白质和RNA基因组成，包括大、小核糖体RNA、15种蛋白质和20种tRNA基因，并且含有两个内含子。值得注意的是，它还包含三种与任何已知蛋白质均无明显同源性的假设蛋白。所有结构基因都位于一条链上，并且显然是沿一个方向转录的。黑曲霉的完整线粒体基因组将有助于未来对曲霉属物种的遗传学、进化和临床鉴定的研究。
