Forsman C A, Pinto da Silva P
Membrane Biology Section, NIH/NCI-FCRF, Frederick, Maryland 21701.
J Cell Sci. 1989 Mar;92 ( Pt 3):415-26. doi: 10.1242/jcs.92.3.415.
We have used fracture-flip to produce new, macromolecular-resolution images of the surface of boar spermatozoa. Over the head, acrosomal and postacrosomal regions display sharply demarcated, subtly different surface textures. The rim is particle-poor, as well as a region above the oblique cords over the posterior ring. The tail shows two morphologically distinct domains: (1) the principal piece is covered by a high density of parallel-helical strands and a high density of large globular particles; (2) the midpiece and the neck are covered by smaller particles with apparent random distribution. Rectangular surface specializations frequently seen near the annulus display a waffle-like texture. With the notable exception of the parallel-helical strands of the principal piece the fracture-flip images of the boar spermatozoon can be directly related to the freeze-fracture morphology of its plasma membrane.
我们利用断裂翻转技术获得了公猪精子表面新的、大分子分辨率的图像。在头部上方，顶体和顶体后区域呈现出界限分明、略有不同的表面纹理。边缘以及后环上方斜索上方的区域颗粒较少。尾部显示出两个形态上不同的区域：（1）主段覆盖着高密度的平行螺旋链和高密度的大球状颗粒；（2）中段和颈部覆盖着随机分布的较小颗粒。在环附近经常可见的矩形表面特化呈现出华夫饼状纹理。除了主段的平行螺旋链外，公猪精子的断裂翻转图像与其质膜的冷冻断裂形态直接相关。
