Huang Xi, Li Guo-chun, Yin Lian, Zhang Zi-han, Liang Yi-xin, Chen Hai-bo
Yao Xue Xue Bao. 2015 Jan;50(1):86-93.
In order to clarify material basis of effective parts of liangxue tongyu prescription, blood-heat and blood-stasis rat model induced by dry yeast was established. The changes of rectal temperature, blood viscosity and plasma viscosity were used to evaluate the cooling-blood and activating-blood effects of liangxue tongyu prescription and its parts. Compared with the model group, the extract from liangxue tongyu prescription, its volatile oil and n-butanol part could significantly reduce rectal temperature (P<0.01), and also reduce blood viscosity and plasma viscosity to various degrees (P<0.01 or P<0.05). So volatile oil and n-butanol part were primarily identified as effective parts of liangxue tongyu prescription. By using GC-MS with normalization method of area to analyze volatile oil of liangxue tongyu prescription, 70 compounds were identified, accounting for about 92.54%, mainly as β-asarone, paeonol, α-asarone and shyobunone. 42 compounds such as peony glycosides, tannins, and iridoid glycosides were identified by HPLC-MS techniques and standard comparison. The study determined the effective parts of liangxue tongyu prescription and clarified the chemical composition providing the foundation for further studies on material basis of liangxue tongyu prescription.
为阐明凉血通瘀方有效部位的物质基础，建立了干酵母致血热血瘀大鼠模型。采用大鼠直肠温度、全血黏度及血浆黏度的变化来评价凉血通瘀方及其各部位的凉血化瘀作用。与模型组比较，凉血通瘀方提取物及其挥发油部位、正丁醇部位能显著降低大鼠直肠温度（P<0.01），并不同程度降低全血黏度及血浆黏度（P<0.01或P<0.05）。初步确定挥发油部位及正丁醇部位为凉血通瘀方的有效部位。采用气相色谱-质谱联用（GC-MS）面积归一化法对凉血通瘀方挥发油进行分析，共鉴定出70个化合物，占挥发油总量的92.54%左右，主要成分为β-细辛醚、丹皮酚、α-细辛醚、顺式甲基异丁香酚等。采用高效液相色谱-质谱联用（HPLC-MS）技术结合对照品对照，鉴定出芍药苷、鞣质、环烯醚萜苷类等42个化合物。本研究确定了凉血通瘀方的有效部位，阐明了其化学成分，为进一步研究凉血通瘀方的物质基础奠定了基础。
