Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; Chengdu Institution of Chinese Herbal Medicine, Chengdu 610016, China.
Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
J Ethnopharmacol. 2019 Apr 24;234:68-75. doi: 10.1016/j.jep.2018.09.035. Epub 2018 Sep 27.
Curcuma Longa Radix (Huangsiyujin in Chinese, HSYJ) has been used in clinical for thousands of years as a traditional Chinese medicine (TCM) and has the effects of invigorating the blood circulation, relieving pain, promoting qi circulation and relieving depression, besides, it is able to clear heart heat and cool blood and cure jaundice. The study aims to determine the active fractions of HSYJ for promotion of blood circulation and relief of pain, and to identify their chemical constituents.
HSYJ was extracted by the systematic solvent method. The effects of promoting blood circulation and relieving pain were determined by measuring pain threshold (hot-plate test), latency of twisting and writhing times (acetic acid induced writhing test), and hemorheology of mice. Chemical constituents of the extractive fractions were analyzed by Gas Chromatography-Mass Spectrometer (GC-MS) or High Performance Liquid Chromatography (HPLC).
The results showed that five fractions (volatile oil fraction, petroleum ether fraction, ethyl acetate fraction, 1-butanol fraction and water fraction) all could increase pain threshold, prolong latency of twisting, decrease the writhing times and influence hemorheology. However, the effects of the volatile oil fraction and ethyl acetate fraction were better than other fractions. Three constituents, namely bisdesmethoxycurum, desmethoxycurumin and curcumin were identified from the active fractions by comparing with standard preparation through HPLC analysis. Forty-five compounds including aR-Turmerone, Curlone, Tumerone, Cyclohexene,1-methyl-4-(1-methylethylidene)-, trans-Sesquisabinene hydrate, (E)-Atlantone, α-curcumene and Zingiberene were identified from the volatile oil fraction by GC-MS.
HSYJ possessed the effect of promoting blood circulation and relieving pain. Curcumins and their derivatives were the major constituents and might be the main bioactive ingredients for the promotion of blood circulation and relief of pain. The study provided evidence and formed a basis for the establishment of HSYJ comprehensive quality evaluation system related to traditional efficacy. It is beneficial to the quality control of HSYJ and its proprietary Chinese medicine.
姜黄（HSYJ，中药名为黄丝郁金）作为一种传统中药，已经在临床上使用了数千年，具有活血化瘀、止痛、行气解郁、清心凉血、治疗黄疸等功效。本研究旨在确定 HSYJ 促进血液循环和缓解疼痛的有效部位，并鉴定其化学成分。
采用系统溶剂法提取 HSYJ。通过测量热板试验的疼痛阈值、扭体和翻滚次数潜伏期（醋酸诱导翻滚试验）以及小鼠血液流变学，确定促进血液循环和缓解疼痛的作用。采用气相色谱-质谱联用仪（GC-MS）或高效液相色谱（HPLC）分析提取物的化学成分。
结果表明，五种馏分（挥发油馏分、石油醚馏分、乙酸乙酯馏分、正丁醇馏分和水馏分）均能提高疼痛阈值、延长扭体潜伏期、减少扭体次数、影响血液流变学。然而，挥发油馏分和乙酸乙酯馏分的效果优于其他馏分。通过 HPLC 分析与标准品比较，从活性馏分中鉴定出三种成分，即双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素。通过 GC-MS 从挥发油馏分中鉴定出 45 种化合物，包括 aR-姜烯、姜烯酮、姜酮、环己烯，1-甲基-4-（1-甲基亚乙基）-、反式-倍半水芹烯、（E）-阿替酮、α-姜黄烯和姜烯。
HSYJ 具有促进血液循环和缓解疼痛的作用。姜黄素及其衍生物是主要成分，可能是促进血液循环和缓解疼痛的主要生物活性成分。该研究为建立与传统功效相关的 HSYJ 综合质量评价体系提供了依据和基础，有利于 HSYJ 及其中药制剂的质量控制。
