Caprioli R M, Moore W T, Martin M, DaGue B B, Wilson K, Moring S
Analytical Chemistry Center, University of Texas Medical School, Houston 77030.
J Chromatogr. 1989 Oct 20;480:247-57. doi: 10.1016/s0021-9673(01)84292-8.
Combined capillary zone electrophoresis (CZE)-continuous-flow fast atom bombardment (CF-FAB) mass spectrometry is described for the analysis of mixtures of peptides. A 90 cm x 50 microns I.D. fused-silica capillary column was used for electrophoretic separations and was connected to the CF-FAB probe via an interface which allows a total flow into the mass spectrometer of about 5 microliters/min. Solutions of peptides were pneumatically loaded onto the CZE capillary, providing sample amounts of 0.1-20 pmol. The magnetic mass spectrometer was scanned over the desired mass range, usually between m/z 500 and 2500. Results are shown for separation and analysis of mixtures of synthetic peptides and also for protease digests of recombinant human growth hormone and horse heart cytochrome c.
本文描述了一种联用毛细管区带电泳（CZE）-连续流快原子轰击（CF-FAB）质谱法分析肽混合物的方法。使用一根内径为50微米、长90厘米的熔融石英毛细管柱进行电泳分离，并通过一个接口将其连接到CF-FAB探头，该接口使进入质谱仪的总流速约为5微升/分钟。肽溶液通过气动方式加载到CZE毛细管上，进样量为0.1 - 20皮摩尔。在所需的质量范围内对磁性质谱仪进行扫描，通常在m/z 500至2500之间。展示了合成肽混合物的分离和分析结果，以及重组人生长激素和马心细胞色素c的蛋白酶消化产物的分析结果。
