Moseley M A, Deterding L J, Tomer K B, Jorgenson J W
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
J Chromatogr. 1989 Oct 20;480:197-209. doi: 10.1016/s0021-9673(01)84289-8.
The coaxial continuous-flow fast atom bombardment (FAB) system has proven to be very useful for interfacing capillary liquid chromatography and capillary zone electrophoresis (CZE) with sector mass spectrometry (MS). The interface can be used for the acquisition of both MS and MS-MS spectra from femtomole levels of non-volatile and/or thermally labile analytes while maintaining separation efficiencies of hundreds of thousands of plates. The use of coaxial fused-silica capillary columns to independently deliver the microcolumn effluent and the FAB matrix to the tip of the FAB probe offers the following advantages: the composition and flow-rates of the two liquid streams can be independently optimized; the FAB matrix does not effect the microcolumn separation process; peak broadening is minimized since the two liquid streams do not mix until they reach the tip of the FAB probe where ion desorption occurs; and, with CZE, active electrophoretic transport delivers the analytes directly to the FAB probe tip. These features combine to make this coaxial continuous flow fast atom bombardment interface particularly well suited for use with microcolumn separation methods.
同轴连续流快原子轰击（FAB）系统已被证明在将毛细管液相色谱和毛细管区带电泳（CZE）与扇形质谱（MS）联用方面非常有用。该接口可用于从飞摩尔水平的非挥发性和/或热不稳定分析物中获取MS和MS-MS光谱，同时保持数十万理论塔板数的分离效率。使用同轴熔融石英毛细管柱独立地将微柱流出物和FAB基质输送到FAB探针尖端具有以下优点：两种液流的组成和流速可以独立优化；FAB基质不会影响微柱分离过程；由于两种液流在到达发生离子解吸的FAB探针尖端之前不会混合，因此峰展宽最小化；并且，对于CZE，主动电泳传输将分析物直接输送到FAB探针尖端。这些特性相结合，使得这种同轴连续流快原子轰击接口特别适合与微柱分离方法一起使用。
