Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, PR China.
Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang, 550025, PR China.
Regul Toxicol Pharmacol. 2018 Dec;100:72-79. doi: 10.1016/j.yrtph.2018.10.015. Epub 2018 Oct 22.
The residue behavior and dietary intake risk of two fungicides (dimethomorph and pyraclostrobin) in grape (Vitis vinifera L.) were investigated from field trials. A modified quick, easy, cheap, effective, rugged, and safe method for simultaneously determining dimethomorph and pyraclostrobin residues in grape and soil was established using high performance liquid chromatography-tandem mass spectrometry. The average recoveries of dimethomorph and pyraclostrobin in the grape and soil matrices varied from 76.88% to 97.05%, with relative standard deviations of 1.73%-10.38%. The degradation half-lives of dimethomorph and pyraclostrobin were 7.3-12.0 days and 3.6-7.0 days in grape and soil, respectively. The terminal residues of dimethomorph and pyraclostrobin in the two matrices were 0.05-0.87 mg/kg. For dietary exposure risk assessments, all of the hazard quotient and hazard quotient index values were below 100%, which indicated that the suspending agents of dimethomorph and pyraclostrobin were sprayed on grape at the recommended dosages with no significant potential risks for Chinese consumers. This study provides a reference for analytically evaluating residual degradation behavior and dietary intake risk of two fungicides under field conditions.
本研究通过田间试验，调查了两种杀菌剂（烯酰吗啉和吡唑醚菌酯）在葡萄（Vitis vinifera L.）中的残留行为和膳食摄入风险。采用高效液相色谱-串联质谱法，建立了一种同时测定葡萄和土壤中烯酰吗啉和吡唑醚菌酯残留的改良型快速、简便、廉价、有效、耐用和安全的方法。烯酰吗啉和吡唑醚菌酯在葡萄和土壤基质中的平均回收率为 76.88%-97.05%，相对标准偏差为 1.73%-10.38%。烯酰吗啉和吡唑醚菌酯在葡萄和土壤中的降解半衰期分别为 7.3-12.0 天和 3.6-7.0 天。两种基质中烯酰吗啉和吡唑醚菌酯的最终残留量为 0.05-0.87mg/kg。在膳食暴露风险评估中，烯酰吗啉和吡唑醚菌酯的危害商和危害指数值均低于 100%，这表明烯酰吗啉和吡唑醚菌酯的悬浮剂以推荐剂量施用于葡萄时，对中国消费者没有显著的潜在风险。本研究为分析评估两种杀菌剂在田间条件下的残留降解行为和膳食摄入风险提供了参考。
