通过检测肠毒素B快速筛查金黄色葡萄球菌感染
Rapid screening for Staphylococcus aureus infection by measuring enterotoxin B.
作者信息
Hosotsubo K K, Hosotsubo H, Nishijima M K, Nishimura M, Taenaka N, Yoshiya I
机构信息
Intensive Care Unit, Osaka University Hospital, Japan.
出版信息
J Clin Microbiol. 1989 Dec;27(12):2794-8. doi: 10.1128/jcm.27.12.2794-2798.1989.
We developed a simple, sensitive laser nephelometric assay (LNA) to detect circulating staphylococcal enterotoxin B (SEB). This assay yields the result within 2 h and needs no special treatment. Influence of protein A, a product generated by Staphylococcus aureus, was negligible in this assay. The levels of SEB in plasma were measured in 28 patients with and without S. aureus infection with the LNA. The levels of SEB in plasma increased significantly in patients with S. aureus infection. We also demonstrated that the levels of SEB in plasma were higher in patients with methicillin-resistant S. aureus infection than in patients with nonresistant staphylococcal infection. Our data indicate that LNA is a useful assay in the early diagnosis of methicillin-resistant S. aureus infection.
我们开发了一种简单、灵敏的激光散射比浊法（LNA）来检测循环中的葡萄球菌肠毒素B（SEB）。该检测方法在2小时内即可得出结果，且无需特殊处理。金黄色葡萄球菌产生的蛋白A在该检测中的影响可忽略不计。我们用LNA对28例有或无金黄色葡萄球菌感染的患者血浆中的SEB水平进行了检测。金黄色葡萄球菌感染患者血浆中的SEB水平显著升高。我们还证明，耐甲氧西林金黄色葡萄球菌感染患者血浆中的SEB水平高于非耐药葡萄球菌感染患者。我们的数据表明，LNA在耐甲氧西林金黄色葡萄球菌感染的早期诊断中是一种有用的检测方法。
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