Grasso Luigino, Wyss Romain, Weidenauer Lorenz, Thampi Ashwin, Demurtas Davide, Prudent Michel, Lion Niels, Vogel Horst
Laboratory of Physical Chemistry of Polymers and Membranes, Ecole Polytechnique Fédérale de Lausanne, Station 6, 1015, Lausanne, Switzerland.
Anal Bioanal Chem. 2015 Jul;407(18):5425-32. doi: 10.1007/s00216-015-8711-5. Epub 2015 Apr 30.
We report on a generic method to detect and identify the molecular profile of exosomes either derived from cultured cell lines or isolated from biofluids. Exosomes are nanovesicles shed by cells into their microenvironment and carry the molecular identity of their mother cells. These vesicles are actively involved in intercellular communication under physiological conditions and ultimately in the spread of various diseases such as cancer. As they are accessible in most biofluids (e.g., blood, urine, or saliva), these biological entities are promising tools for cancer diagnostics, offering a non-invasive and remote access to the molecular state of the disease. The composition of exosomes derived from cancer cells depends on the sort and state of the tumor, requiring a screening of multiple antigens to fully characterize the disease. Here, we exploited the capacity of surface plasmon resonance biosensing to detect simultaneously multiple exosomal and cancer biomarkers on exosomes derived from breast cancer cells. We developed an immunosensor surface which provides efficient and specific capture of exosomes, together with their identification through their distinct molecular profiles. The successful analysis of blood samples demonstrated the suitability of our bioanalytical procedure for clinical use.
我们报告了一种通用方法，用于检测和识别源自培养细胞系或从生物流体中分离出的外泌体的分子谱。外泌体是细胞释放到其微环境中的纳米囊泡，携带其母细胞的分子特征。这些囊泡在生理条件下积极参与细胞间通讯，并最终参与各种疾病（如癌症）的传播。由于它们存在于大多数生物流体（如血液、尿液或唾液）中，这些生物实体是癌症诊断的有前途的工具，提供了对疾病分子状态的非侵入性和远程访问。源自癌细胞的外泌体的组成取决于肿瘤的类型和状态，需要筛选多种抗原以全面表征疾病。在这里，我们利用表面等离子体共振生物传感技术，同时检测源自乳腺癌细胞的外泌体上的多种外泌体和癌症生物标志物。我们开发了一种免疫传感器表面，可对外泌体进行高效、特异性捕获，并通过其独特的分子谱进行识别。对血样的成功分析证明了我们的生物分析程序适用于临床应用。
