Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, 4072, Australia.
Biomedical Institute for Global Health Research & Technology, National University of Singapore, Singapore, 119228, Singapore.
Anal Bioanal Chem. 2019 Mar;411(7):1311-1318. doi: 10.1007/s00216-019-01608-5. Epub 2019 Feb 5.
The development of a sensitive and specific detection platform for exosomes is highly desirable as they are believed to transmit vital tumour-specific information (mRNAs, microRNAs, and proteins) to remote cells for secondary metastasis. Herein, we report a simple method for the real-time and label-free detection of clinically relevant exosomes using a surface plasmon resonance (SPR) biosensor. Our method shows high specificity in detecting BT474 breast cancer cell-derived exosomes particularly from complex biological samples (e.g. exosome spiked in serum). This approach exhibits high sensitivity by detecting as low as 8280 exosomes/μL which may potentially be suitable for clinical analysis. We believe that this label-free and real-time method along with the high specificity and sensitivity may potentially be useful for clinical settings.
开发一种敏感且特异的外泌体检测平台是非常需要的，因为人们相信外泌体可以将重要的肿瘤特异性信息（mRNA、miRNA 和蛋白质）传递到远处的细胞，从而进行二次转移。在此，我们报道了一种使用表面等离子体共振（SPR）生物传感器实时、无标记检测临床相关外泌体的简单方法。我们的方法在检测 BT474 乳腺癌细胞衍生的外泌体时具有很高的特异性，特别是在复杂的生物样本（如外泌体掺入血清中）中。该方法通过检测低至 8280 个/μL 的外泌体表现出高灵敏度，这可能适用于临床分析。我们相信，这种无标记和实时的方法，以及高特异性和灵敏度，可能对外泌体的临床研究具有重要意义。
