Kiyozuka Y, Noda T, Adachi S, Adachi S, Akada S, Ichijo M
Department of Obstetrics and Gynecology, Nara Medical University.
Nihon Sanka Fujinka Gakkai Zasshi. 1989 Dec;41(12):1951-8.
Ascites fluid, chest effusion, cyst fluid, and serum mainly obtained from malignant-ovarian-cancer patients were applied to quantitate the concentration of the tumor marker CA125. The same procedure was also performed for ascites, cyst fluid, and serum mainly obtained from benign-ovarian-tumor patients. To know how CA125 proceeds from tumor cells into the circulation, a CA125-producing, ovarian-cancer-cell line (SHIN-3) was transplanted sub-cutaneously into nude mice. Then the relationship between the grown tumor size and CA125 concentration in serum was analyzed and the histo-pathological background was also evaluated. Quantitative analysis of the ascites and cyst fluid showed no remarkable difference between the malignant-tumor patients and benign ones: however, the CA125 concentration in serum of malignant-tumor patients was up to 90 times higher than in benign ones. The proceeding ratio of CA125 in its productive site (cyst fluid, for example) to that in the serum of patients with malignant lesions was 24 times as high as with benign lesions. No relationship between tumor sizes and the CA125 concentration in serum in nude mice was observed. And in the mice which had high CA125 in serum, the capsules around the tumor tended to be destroyed and vessel infiltration by tumor cells was remarkable.
收集主要来自恶性卵巢癌患者的腹水、胸腔积液、囊肿液和血清，用于定量肿瘤标志物CA125的浓度。对主要来自良性卵巢肿瘤患者的腹水、囊肿液和血清也进行同样的操作。为了解CA125如何从肿瘤细胞进入循环系统，将一株产生CA125的卵巢癌细胞系（SHIN-3）皮下移植到裸鼠体内。然后分析肿瘤生长大小与血清中CA125浓度之间的关系，并评估组织病理学背景。腹水和囊肿液的定量分析显示，恶性肿瘤患者和良性肿瘤患者之间无显著差异：然而，恶性肿瘤患者血清中的CA125浓度比良性肿瘤患者高90倍。恶性病变患者的CA125在其产生部位（如囊肿液）与血清中的比值是良性病变患者的24倍。未观察到裸鼠肿瘤大小与血清中CA125浓度之间的关系。并且在血清中CA125含量高的小鼠中，肿瘤周围的包膜往往被破坏，肿瘤细胞的血管浸润明显。
